In this study, we utilized the decellularized extracellular matrix (ECM) under the ultrasonication to obtain the ultrasonic decellularized ECM (UdECM). In SDS-PAGE studies, the results demonstrated that UdECM was mainly composed of type I collagen. The mechanical strength increased with the increase of concentration of UdECM in rheology studies. In addition, the scanning electron microscope (SEM) images showed the highly porous structures, which can absorb the large amount of fluid and cells attached. In diabetic wound healing study, the PRP-loaded UdECM and sacchachitin (SC) hydrogel exhibited the synergistic wound healing on the 14th day. Based on the results, PRP-loaded SC and UdECM showed great potential in future clinical applications.