Speaker
Description
From 2020's WHO data, Ischemic heart disease is already the world's leading cause of death, and it also brings a high burden to the world. Nowadays, almost all clinical therapies based on supportive therapies, not is curative therapies, which cannot regenerate myocardial tissue. If using heart transplantation, there has not been resolved yet that donated hearts will be little supply and the problem of high mortality. Moreover, cell therapy has become an emerging direction for myocardial regeneration in recent years, which uses a thermo-sensitive polymer pNIPAAm as a cultural matrix. Furthermore, Japan's medical market already has a local team listed its cell therapy product. On the other hand, we have been committed to developing new cell sheet engineering technology for regenerative medicine for a long time. In our design for myocardial cell sheet, the plasma-activated surface chemical modification was used to graft the combination of disulfide bond-containing amino acid and biopolymer of cell feed onto a porous and transparent PET membrane be a culture matrix. Furthermore, spontaneously reductive cleaving the disulfide bond with a reducing amino acid addition can achieve the cell sheet detachment mechanism. Briefly, after surface chemical modification, the contact angle measurement and Electron Spectroscopy for Chemical Analysis (ESCA) were used to confirm the change in the chemical structure of grafted molecules in the disulfide bond cut period in this study. Then, it was successfully prepared to a culture insert as a culture device by ultrasonic welding between PET membrane and culture insert frame, and then the myocardium and leg muscles for the reconstruction of myocardial tissue were cultivated. As far as the results are concerned, we have isolated and amplified myocardial and skeletal muscle progenitor cells from New Zealand white rabbits successfully prepared cell sheets of these two tissues and confirmed the correctness of the obtained cell sheets by immunocytochemical staining. It can say that we have confirmed that the new system for cell sheet engineering is feasible. We will use New Zealand white rabbits with chronic Ischemic cardiomyopathy (ICM) formed by an Ameroid constrictor and then reconstruct its myocardium with an autologous myocardial or skeletal muscle precursor cell sheet. We hoped to be applied to clinical myocardial reconstruction as soon as possible in the near few years.
52354506488
