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Description
Polysaccharide-based hydrogels have been developed in the fabrication of multifunctional microenvironments for tissue fabrication. In this study, two opposite charged polysaccharides including chitosan and hyaluronic acid conjugated with phenol moieties (PH) (CH-PH &HA-PH respectively were mixed at different ratios and utilized to enzymatic cross-linking in the presence of H2O2 as an electron donor to make hybrid hydrogel scaffolds for cartilage tissue engineering. The physical characteristics of hydrogels including gelation time and water contact angle were measured and results showed decreased these properties with increasing HA-PH content. Meanwhile, the compressive modulus and strength of the hydrogels increased with the amount of HA-PH. The cellular studies showed proper cell viability and proliferation for cells on optimum hydrogel surface (CHA-PH2) compared with neat hydrogels. The MSCs were able to undergo robust chondrogenesis and generate the proper cartilage scaffolds in hybrid CH-PH with optimum concentration of HA-PH named CHA-PH2 indicated by the real-time polymerase chain reaction of Col2, SOX9, and ACAN, and mechanical testing suggesting its potential in future in vivo model for the repair of cartilage defects. In summary, the results demonstrated the applicability of developed polysaccharide-based hydrogel in cartilage tissue engineering.
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