Speaker
Description
Adipose tissue plays a crucial role in the human body including heat regulation, energy and hormone homeostasis. The clinical need of adipose tissue is mainly found in reconstructive and plastic surgery, e.g. after tumor resection or to treat deep wounds. The aim of tissue engineering is to build-up vital and functional 3D-tissue models in vitro. Furthermode, adipose tissue is an important part of cultured meat in food science. For both applications it is important to optimize the culture condition of primary adipose dericed stem cells (ASCs) and mature adipoctes (ACs).
In the last years we optimized numerous aspects for gaining a reproducible and physiological adipose cell and tissue culture. In detail we developed defined media for culture, differentiation and long term culture, analyzed different biomaterials for 3D-setup and evaluated usability as bioinks in bioprinting. Further, we validated different coculture setups e.g. in combination with endothelial or immune cells. We successfully generated spheroid cultures with ASCs. Moreover 3D tissue models were build-up manually or by using bioprinting techniques.
Viability, differentation and function of the ASCs and ACs were evaluated by different stainings, ELISAs, quantitative assays (leptin, glycerol and western blots after severeal days and weeks of culture. In addition, we tested different biopolymers as scaffold material for 3D-adipose tissue models such as gelatin, collagen, gellan gum etc.. In other studies we used in vitro cell derived ECM produced by ASCs for getting improved culture conditions.
In summary, we have developed optimized culture and differentation protocols (like setup of defined media) for 2D and 3D culture ASCs and ACs as well as cocultures with other cells. For bioprinting of the 3D-models tailor-made bioinks were developed. We were able to generate different setups of functional 3D-adipose tissue models and maintained their functionality over several weeks.
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