Speaker
Description
TITLE: A study on hBM-MSCs chondrogenic commitment by 3D Collagen scaffold loaded with PLGA nanocarriers for TGF-β1 controlled release
AUTHORS: Lamparelli Erwin P.1, Lovecchio J.2, Giordano E.2, Maffulli N.1, Della Porta G. 1,3(*),
1 Department of Medicine, Surgery and Dentistry, University of Salerno, Via S. Allende, Baronissi (SA), Italy
2 Department of Electrical, Electronic and Information Engineering “Guglielmo Marconi” (DEI), University of Bologna, Via dell’Università 50, Cesena (FC), Italy.
3 Research Centre for Biomaterials BIONAM, Università di Salerno, via Giovanni Paolo II, 84084 Fisciano (SA), Italy.
(*) Speaker at oral presentation.
Introduction: Treatment of hyaline cartilage lesions remains a major challenge owing to the avascular nature of itself, which prevents spontaneous healing. To date the use of Mesenchymal Stem Cells (MSC) and tissue engineering protocols represents an alternative strategy and offers great potential to improve joint therapy. The development of injectable 3D scaffold able to stimulate the formation of a new cartilaginous tissue is crucial to the success of the therapeutic strategy. Despite collagen-based hydrogel lack of the appropriate mechanical resistance it has been described as excellent matrix to support the chondrocyte and stem cells growth because are more like natural ECM [1,4].
Methods: A 3D collagen scaffold was assembled as biomimetic extracellular matrix for human Bone Marrow Mesenchymal Stem Cells (hBM-MSC) induction towards chondrogenic phenotype by dispersing poly-lactic-co-glycolic acid (PLGA) nano-carriers, carrying and delivering Transforming Growth Factor (hTGF- b1) payload, within the 3D structure. Cultures were performed both in static than dynamic condition (perfusion).
Results: hBM-MSCs early commitment towards a chondrogenic phenotype was confirmed both via immuno-histology that revealed the type II collagen fiber deposition within the scaffold and by q-RT-PCR that indicated the increase of gene expression of SOX9 and COL2A1, after 16 days of culture. The dynamic culture by perfusion assured a better mass exchange and more effective scaffold matrix reshaping.
Discussion and Conclusions: The data suggested the effectiveness of 3D collagen scaffold carrying PLGA microspheres for drug delivery as injectable scaffold and opened perspectives for its use as advanced therapy device to promote cartilage regeneration.
References
Lamparelli E.L., Lovecchio J., Ciardulli M.C. (…) Della Porta G., Chondrogenic Commitment of Human Bone Marrow Mesenchymal Stem Cells in a Perfused Collagen Hydrogel Seeded with hTGF-β1-Releasing PLGA Microcarrier, Pharmaceutics, 2021, 13(3), 399.
Palazzo, I., Lamparelli, E.P., Ciardulli, M.C. (...) Della Porta, G., Supercritical emulsion extraction fabricated PLA/PLGA micro/nano carriers for growth factor delivery: Release profiles and cytotoxicity, International Journal of Pharmaceutics, 2021, 592, 120108
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