"Introduction: Severe skin lesions can be life-threatening conditions, especially in burnt patients. We recently described the UGRSKIN model of bioartificial human skin generated by tissue engineering as an ATMP1. Although preliminary clinical results are promising, the biofabrication procedure requires long periods of time due to the need of obtaining abundant keratinocyte cell populations from a single biopsy obtained from healthy skin areas. Reduction of the biofabrication time could contribute to an early treatment of burnt patients in critical risk. One of the methods used to increase the efficiency of keratinocyte culturing is the use of bioactive molecules able to induce cell proliferation. In this milieu, maslinic acid is a bioactive compound found in olive oil which has multiple benefits for health2. This molecule has been widely tested for different purposes, but its potential role in keratinocyte-based tissue engineering has not been determined to the date.
Methodology: In this study, we cultured human keratinocytes using a culture medium enriched with increasing concentrations of maslinic acid. Then, we analyzed cell viability and proliferation using several biochemical and metabolic methods.
Results: Results showed that maslinic acid was very safe for the cells, and concentrations associated to a decrease of cell viability were very high. However, the lowest concentrations resulted in increased cell viability and proliferation of human keratinocytes, with higher number of cells found at the concentrations of 1-5µg/mL.
Conclusions: These preliminary results point out the putative beneficial effects of maslinic acid to accelerate and improve the biofabrication process of human bioartificial skin."