Scaffolds based on tricalcium phosphate and bacteria-derived polyhydroxyoctanoate – cytocompatibility studies

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ICE Krakow

ICE Krakow

ul. Marii Konopnickiej 17 30-302 Kraków


Cichoń, Ewelina (Faculty of Materials Science and Ceramics, AGH University of Science and Technology )


Tissue engineering faces a major challenge in providing novel, functional materials that will meet the requirements of tissue engineering. These properties can be provided by tricalcium phosphate-based bioceramics (TCP). The combination of a ceramic matrix with polymers creates great opportunities to improve the physicochemical and biological properties of the obtained composites. Polyhydroxyoctanoate (PHO) belongs to the polyhydroxyalkanoates (PHAs) family. Due to its degradation products – nourishing (R)-3-hydroxyacids[1], the application of this novel elastomeric polymer opens new routes for promoting tissue regeneration.
In this study, the bioceramic/polymer based scaffolds (TCP/PHO) were prepared as previously described[2]. To assess the possibility of using TCP/PHO composites as bone tissue substitutes, their biocompatibility was tested using MC3T3-E1 mouse preosteoblastic cell line. Cells were cultured in Minimal Essential Medium Eagle medium with alpha modification - α-MEM supplemented with 10% fetal bovine serum - FBS and antibiotics: penicillin/streptomycin at 37°C at 5% CO2 concentration in an incubator. Materials’ samples were incubated in α-MEM for 24 hours at 37°C in 5% CO2. MC3T3-E1 cells were seeded in a 96-well plate at 25·103 cells per well and incubated in 150 µl α-MEM for 24 hours. Then, the media was exchanged with extracts obtained by incubating material samples in α-MEM. Cells were cultured for 24 hours. The negative control was cells cultured in medium alone (MEM). The cell viability assay (Alamar Blue) was performed.
In the case of supernatants from TCP scaffolds, cell viability was 117.11 ± 5.59%. which is significantly higher in comparison to the negative control (100% viability was determined for cells cultured in α-MEM medium alone). This result indicates that the extract of TCP material has a beneficial effect on cells during the first 24 hours. The TCP/PHO scaffolds were fully biocompatible and cell viability was comparable to that of cells in α-MEM. Cell survival in extracts of polymer-infiltrated ceramic (TCP/PHO) was 105.15 ± 3.62%. The reason for these differences is the hydrophobic nature of polyhydroxyoctanoate.
The obtained results suggest that both bioceramic and composite scaffolds are fully cytocompatible.

Research funded by the National Center for Research and Development, Poland, grant Techmatstrateg no. TECHMATSTRATEG2/407507/1/NCBR/2019. E.C. acknowledges financial support from the National Science Centre, Poland under Doctoral Scholarship No. 2019/32/T/ST5/00207

[1] Dwivedi, R., Pandey, R., Kumar, S., & Mehrotra, D. (2020). Polyhydroxyalkanoates (PHA): Role in bone scaffolds. Journal of oral biology and craniofacial research, 10(1), 389-392.
[2] Cichoń, E., Haraźna, K., Skibiński, S., Witko, T., Zima, A., Ślósarczyk, A., ... & Guzik, M. (2019). Novel bioresorbable tricalcium phosphate/polyhydroxyoctanoate (TCP/PHO) composites as scaffolds for bone tissue engineering applications. Journal of the Mechanical Behavior of Biomedical Materials, 98, 235-245.


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