Speaker
Description
"CONTROLLING CELL RESPONSES WITH SURFACE POTENTIAL ON ELECTROSPUN POLY(L-LACTIDE) (PLLA) SCAFFOLDS PRODUCED WITH POSITIVE AND NEGATIVE VOLTAGE POLARITY
Martyna Polak1*, Urszula Stachewicz1
1Faculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, Poland
*mpolak@agh.edu.pl
Abstract
Cell responses are essential for all tissue regeneration processes. Interaction between cell and material results from biomaterial surface properties such as geometry, wettability, stiffness, or roughness1.
Most studies focus on the surface topography to control cells, however, another crucial parameter is surface potential2. Electrospinning is a commonly used method to produce polymer fibers with tailored surface properties such as voltage polarity. In electrospinning, fibers are produced by drawing charged jets of polymer solution in an electrostatic field between needle and collector with an applied potential difference. On the surface of the polymer solution and the jet charges (positive or negative) accumulate, which contributes to the reorientation of polymer chains pieces or functional groups by electrostatic interactions3. Surface potential is a significant factor of biomaterials regulating cell adhesion and proliferation. In our research, we use poly(L-lactide) (PLLA) to electrospun scaffolds using positive and negative voltage polarity to control their surface potential.
The properties of fibers are verified with scanning electron microscopy (SEM), surface potential with Kelvin probe force microscopy (KPFM), and compared with zeta potential measurements. Finally, the produced scaffolds were used in the osteoblast cell culture (MG-63 cell line) studies and analyzed with confocal microscopy. The result show possibility to control anchoring and proliferation of cells in PLLA. Manufactured with positive and negative voltage polarity to tune their surface potential.
Acknowledgments
The study was conducted within the funding from OPUS 17 project granted by National Science Centre in Poland, No 2019/33/B/ST5/01311.
References
1. Metwally, S. & Stachewicz, U. Surface potential and charges impact on cell responses on biomaterials interfaces for medical applications. Materials Science and Engineering C vol. 104 (2019).
2. Metwally, S. et al. Surface potential and roughness controlled cell adhesion and collagen formation in electrospun PCL fibers for bone regeneration. Materials and Design 194, (2020).
3. Ura, D. P. et al. The role of electrical polarity in electrospinning and on the mechanical and structural properties of as-spun fibers. Materials 13, (2020)."
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