Purpose/Objectives: Bioactuator is a structure that responds to external stimuli by combining an artificial flexible structure and a living biosystem (cells and tissues). Here, we generated and experimented with a structure whose movement can be controlled by external signals by attaching myoblasts to an elastic material in the form of a micro-wire for a bioactuator.
Methodology: Micro-wire with three or six hundred micrometer diameter was generated using various ratios of curing agent with polydimethylsiloxane (PDMS). The generated micro-wire was characterized by studying the stiffness, strength, and then myoblasts were adhered and cultured onto the convex surface after surface treatment. The cell-attached microwire was cultured with electrical stimulation for 7 days after the differentiation period.
Results: It was confirmed that myoblasts adhered onto the convex surface of the micro-wire until the end of the experiment. The myofibers were observed with the microwire and aligned morphology. The skeletal muscle-based microwire was showed a higher movement with electrical stimulation in a group of electrical cultures.
Conclusion/Significance: Thus, it has been shown that a simple micro-wire is implemented by electrical stimulation for biohybrid actuator.