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The usage of artificial materials for tissue engineering is well-known. Their use as a vascular prosthesis is being considered more often. One of the most promising materials for use for applications in the cardiovascular system is polyurethane which has a good bio- and hemocompatibility as well as appropriate mechanical properties. However, researchers are still looking for methods to improve it in order to reduce the risk of an unwanted reaction of the human body. The most promising approach is seeding vascular graft with endothelial cells.
The effect of surface modification with REDV and YIGSR peptides on hemocompatibility and competitive adhesion of endothelial and smooth muscle cells was examined. According to the literature, these sequences are supposed to promote endothelial cell adhesion, while not significantly affects the modified material hemocompatibility. Several methods of endothelial cells seeding on the inner surface of cylindrical grafts were investigated. In the next stage of the research, the coculture of endothelial cells and smooth muscle cells was carried out. The last stage of the study was the analysis of the interaction of the tested materials with blood under flow conditions. The number and morphology of the platelets adhering to the surface of the prosthesis were analyzed, as well as their activity (CD62P + platelets).
The analysis of the co-culture results showed that the presence of REDV and YIGSR peptides promoted selective adhesion of endothelial cells, and investigation of the blood-material interaction showed a reduction in the percentage of platelets area on the surface of the peptide-modified prostheses.
This work was supported in part by the National Centre for Research and Development in the frame of Project Contract No LIDER/18/0104/L-8/16/NCBR/2017
This project was funded in part by National Science Centre, Poland, grant number: 2020/39/I/ST5/01131.
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