Evaluation of keratin biomaterial containing silver nanoparticles as a potential wound dressing in full-thickness skin wound model in diabetic mice

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ICE Krakow

ICE Krakow

ul. Marii Konopnickiej 17 30-302 Kraków


Konop, Marek (Department of Experimental Physiology and Pathophysiology, Laboratory of Center for Preclinical Research, Medical University of Warsaw)


"Impaired wound healing in diabetics particularly is a major healthcare problem, and new
therapeutic options are expected. The aim of the study was to evaluate the potential role of insoluble keratin-derived powder containing silver nanoparticles (FKDP-AgNP) in the full-thickness surgical skin wound model in diabetic mice. The scanning electron microscopy image showed that the keratin surface is covered by a single layer of silver nanoparticles. Dynamic light scattering and micellar electrokinetic chromatography showed three fractions of silver nanoparticles with an average diameter of 130, 22.5, and 5 nm. Microbiologic results revealed that the designed insoluble FKDP-AgNP dressing to some extent inhibits the growth of E. coli and S. aureus. In vitro assays showed that the FKDP-AgNP dressing did not inhibit fibroblast growth or induce hemolysis. In vivo studies using a diabetic mice model confirmed biocompatible properties of the insoluble keratin dressings. FKDP-AgNP significantly accelerated wound closure and epithelization at days 5 and 8 (p <0.05) when compared with controls. Histological examination of the inflammatory response documented that FKDP-AgNP-treated wounds contained predominantly macrophages, whereas their untreated variants showed mixed cell infiltrates rich in neutrophils. Wound inflammatory response based on macrophages favors tissue remodeling and healing.Cytokine analysis during wounding showed an increased concentration of IL-10 during the healing course. Statistical significance was observed between Day 8 and Day 15 (p < .05). In conclusion, the investigated FKDP-AgNP dressing consisting of an insoluble fraction of keratin, which is biocompatible, significantly accelerated wound healing in a diabetic mouse model."

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