Speaker
Description
Introduction
First place of interaction between the biological and artificial system is the surface. Surface properties can influence many cellular functions. The efficient way of surface functionalization is thin composite films prepared in situ in colloidal dispersion mode. The combination of intrinsic electrical conductivity with easy preparation and modification procedures of polyaniline (PANI) opens the door for its promising applications.
Methodology
PANI coating films were prepared via the oxidation of aniline hydrochloride with ammonium peroxydisulfate where sodium hyaluronate (SH) or chitosan (CH) were used as stabilizers. Surface energy (measured by acid base method), surface topography (AFM), and conductivity (van der Pauw) of functionalized surfaces were determined. Biological properties such as determination of protein adsorption on the film, and adhesion of mouse embryonic fibroblast cell line NIH/3T3 were investigated. The antibacterial activity of the films against E. coli and S. aureus was also tested.
Results
The results of surface energy indicate that the addition of biopolymers (PANI-SH and PANI-CH) changed the surface energy. PANI films without stabilizers showed twice as high conductivity. Films with polysaccharides also showed antimicrobial activity against E. coli. Compared to the reference sample, protein adsorption increased only in case of unstabilized PANI and PANI-SH. Mouse fibroblast cells were capable of adhesion on PANI films stabilized by SH and CH.
Conclusion
PANI films itself do not provide an appropriate cell response. On the other hand, PANI films stabilized by SH and CH improve their biological properties. Therefore, the presence of bioactive polysaccharides enhances the applicability of PANI in tissue engineering and biomedicine.
Acknowledgments
This work was supported by OP RDE by the project The Development of Capacity for Research and Development of TBU in Zlín - CZ.02.2.69/0.0/0.0/16_028/0006243
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