Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Chapter Conference 2022

Session

S12 Biofabrication with light-based technologies and high-definition printing

S12

Jun 29, 2022, 11:00 AM

ICE Krakow

Conveners

S12 Biofabrication with light-based technologies and high-definition printing

• Tiziano Serra
• Marcy Zenobi-Wong

949. Light-driven technologies to steer the functionality of volumetric engineered tissues and organoids

Levato, Riccardo (Utrecht University)

6/29/22, 11:00 AM

invited lecture

Organ- and tissue-level biological functions are intimately linked to microscale cell-cell interactions and to the overarching tissue architecture. Advances in biofabrication technologies offer unprecedented opportunities to capture salient features of tissue composition and thus guide the maturation of engineered constructs into mimicking functionalities of native organs. Light-based...

988. LASER-BASED HIGH-RESOLUTION 3D PRINTING AND BIOPRINTING FOR TISSUE ENGINEERING

Ovsianikov, Aleksandr (3D Printing and Biofabrication Group, TU Wien)

6/29/22, 11:20 AM

invited lecture

Various 3D Printing and Bioprinting approaches have proven useful for tissue engineering applications. The achievable spatial resolution of the most widespread technologies, such as for example extrusion, is usually in the range of hundreds of micrometers, limited by the intrinsic attributes of these methods. However, light-based technologies and in particular multiphoton lithography (MPL) can...

155. HARNESSING MICROFLUIDIC BIOPRINTING TO FABRICATE GRADIENT-LIKE POROUS 3D CONSTRUCTS VIA EMULSION INK DEPOSITION

Marcotulli, Martina (3D Microfluidic Biofabrication laboratory, Center for Life Nano- & Neuro- Science – CLN2S, Italian Institute of Technology)

6/29/22, 11:40 AM

podium presentation

The exceptional properties of natural structures with density gradients (e.g. bone, sponges, bamboo) have stimulated the interest in reproducing such complex architectures harnessing biopolymer functionality. However, the possibility to generate a hierarchical structure comprising multiple density gradient has not yet demonstrated, mainly due to the lack of technological advancements in...

163. BOTTOM-UP TISSUE ENGINEERING BASED ON MICROSCAFFOLDS PRODUCED BY HIGH-RESOLUTION 3D PRINTING

Kopinski-Grünwald, Oliver (3D Printing and Biofabrication Group, Institute of Materials Science and Technology, TU Wien/ Austrian Cluster for Tissue Regeneration)

6/29/22, 11:50 AM

podium presentation

Introduction
While the two most-commonly applied approaches in tissue engineering (TE), namely the scaffold-based and the scaffold-free approach come with individual advantages but also drawbacks, Ovsianikov et al. proposed a third strategy for tissue engineering which combines the advantages of both approaches [1].
We propose here to utilize this third strategy to fabricate millimeter-size...

165. DEFINED-GEOMETRY MICROPARTICLES PRODUCED BY TWO-PHOTON POLYMERISATION FOR SKELETAL APPLICATIONS

Owen, Robert (Regenerative Medicine and Cellular Therapies, School of Pharmacy, University of Nottingham Biodiscovery Institute )

6/29/22, 12:00 PM

podium presentation

Introduction

Bone graft substitutes are typically provided as ceramic granules. Whilst they have undergone successful clinical implementation, they are not without limitations. These include brittleness, variable resorption rates and a lack of control over the microarchitecture, all of which can lead to poor integration of the graft and fibrous tissue formation at the interface. Porous...

164. Microfluidics-assisted bioprinting of double-emulsion droplets

Terrazas Mallea, Ronald (Institute of Physical Chemistry, Polish Academy of Sciences )

6/29/22, 12:10 PM

podium presentation

Introduction
Microfluidic droplet-based bioprinting offers several advantages over conventional extrusion-based bioprinting methods such as (i) high-precision spatial patterning of the biologics (including cells, molecules, drugs and bioinks) and (ii) ease of their compartmentalization. These advantages, combined with high reproducibility of the generated microdroplets, facilitate...

162. EFFECT OF LIGHT STIMULI IN VOLUMETRIC BIOPRINTING ON CELL FUNCTIONALITY AT SINGLE CELL LEVEL

Gueye, Marième (Regenerative Medicine Center Utrecht / University Medical Center Utrecht )

6/29/22, 12:20 PM

podium presentation

INTRODUCTION. Volumetric bioprinting (VBP) is a recently developed light-based biofabrication method enabling the rapid generation of complex 3D structures within seconds. Short printing times combined with freedom of design allow for the advancement of novel in vitro models and physiologically relevant constructs. However, a more in-depth understanding of the effects of light-based...