Conveners
S66 Wanted: Dead or Alive? Quantitative microscopy of spheroid and organoid tissues
- Ruslan I. Dmitriev
- Michael Monaghan (Trinity College Dublin)
Intravital microscopy (IVM) has revolutionized our understanding of single-cell behavior in complex tissues by enabling real-time observation of molecular and cellular processes in their natural environment. In preclinical research, IVM has emerged as a standard tool for mechanistic studies of therapy response and the rational design of new treatment strategies. For understanding immune...
In this study, fluorescence lifetime imaging of NAD(P)H-based cellular autofluorescence is applied as a non-invasive modality to classify two contrasting states of human macrophages by proxy of their governing metabolic state. Macrophages were obtained from human blood-circulating monocytes, polarised using established treatments, and metabolically challenged using small molecules to validate...
"Introduction
Multicellular spheroids can be a powerful model mimicking the physiological environment of the tissue in a microscale format. They are often seen as ‘microscale-bioreactors’ providing an appropriate environment for cell differentiation and stem and cancer cell niche, which makes them important for physiological studies and biofabrication. However, this advantage becomes a...
Introduction
Advanced in vitro models (e.g., organoids) are three-dimensional (3D) constructs usually generated from cells with a degree of stemness. This, accompanied by the multiplicity of parameters which condition organoid growth and morphology, results in constructs of different shapes and size and thereby functional properties. To date, a quantitative framework for robust measurement...
Introduction: Lasers have been used for years in the field of cancer therapy. Nanosecond pulsed lasers, in particular, have been used in the generation of reactive oxygen species via plasma for the triggering of immunogenic cell death[1][2], for the delivery of biomolecules intracellularly via optoporation[3] and for tumor resection[4]. Notwithstanding this large range of uses, the full impact...
