Introduction.
Osteoarthritis (OA) is a highly prevalent musculoskeletal disease characterized by a chronic inflammation and joint disability. OA is characterized by cartilage degradation and bone overgrowth. Current pharmacological approaches have a merely symptomatic effect and do not delay disease progression.
Several studies have pointed out that chondrocyte senesce contribute to the...
Introduction: Bioactive factors contained in Platelet Lysate (PL) promote the isolation and proliferation of Mesenchymal Stem Cells (MSCs) and the growth of cell lines. It is well known that PL not only enhances cell proliferation compared to Fetal Bovine Serum (FBS) without affecting the cell differentiation capability, but it is also able to activate resting quiescent cells to re-enter cell...
Introduction
Cellular senescence is an irreversible cell-cycle arrest program that has been associated with numerous biological processes. While emerging studies have extended its role to tissue repair, the focus has been on the effect of the senescence-associated secretory phenotype (SASP) rather than the direct role of the program beyond its paracrine effect. Using a previously...
Introduction
The skin, as the outermost barrier of the body, is exposed to threats from the external environment including injuries, oxidative stress and UV irradiation. Therefore, efficient systems of repair and antioxidant protection of damaged skin that are achieved by an extraordinary mechanism involving the interaction of cells, cytokines and growth factors are vital for survival. In the...
[Introduction] Fibroblasts are the major cells that consist of connective tissues in animal tissues and are known as the main extracellular matrix (ECM) production cells to maintain the tissue physical properties. Although they are cells that are famous and frequently found in our body, their biological mechanisms, especially the differences from other similar fibroblastic cells are still...
Purpose: To investigate the effect of Losartan on articular chondrocyte hypertrophy in a monolayer culture under chemically induced hypoxia with CoCl2.
Materials & Methods: The 2nd passage of human knee chondrocytes was used to determine the cell viability in response to the treatment with Losartan or CoCl2 in the following concentrations 10-4 – 10-12 M. Chondrocytes were seeded in a...
INTRODUCTION: The question of efficient preservation of cells, especially Mesenchymal stem cells (MSC) becomes more and more urgent due to the impetuous development of cell therapy in clinics. There are two main problems in the improvement of mixtures used for cells cryopreservation. First is the application of DMSO as cryoprotectant, which is known to cause different side effects, and the...
The term "cellular senescence" refers to an irreversible cell cycle arrest that can be caused by a variety of stressors and is accompanied by an accumulation of damage, changes in cell shape and secretory profile, and epigenetic abnormalities in primary cells. There is an accumulation of senescent cells in development, aging and tissue regeneration; all of which are conditions with increased...
