Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Chapter Conference 2022

Session

PS25 Cellular senescence in tissue damage and regeneration

PS25

Not scheduled

ICE Krakow

437. A new strategy to modulate chondrocyte senescence and reduce osteoarthritis progression

Martinez-Borrajo, Rebeca (Universidade de Santiago de Compostela)

poster

Introduction.
Osteoarthritis (OA) is a highly prevalent musculoskeletal disease characterized by a chronic inflammation and joint disability. OA is characterized by cartilage degradation and bone overgrowth. Current pharmacological approaches have a merely symptomatic effect and do not delay disease progression.
Several studies have pointed out that chondrocyte senesce contribute to the...

434. c-MYC pathway modulation in cell activated by platelet lysate stimulation

Nardini, Marta (Biotherapy Laboratory, Department of Internal Medicine (DIMI) University of Genova )

poster

Introduction: Bioactive factors contained in Platelet Lysate (PL) promote the isolation and proliferation of Mesenchymal Stem Cells (MSCs) and the growth of cell lines. It is well known that PL not only enhances cell proliferation compared to Fetal Bovine Serum (FBS) without affecting the cell differentiation capability, but it is also able to activate resting quiescent cells to re-enter cell...

436. EFFECTS OF CELLULAR SENESCENCE ON MECHANOSENSATION: IMPLICATIONS FOR TISSUE REGENERATION

Sohrabi, Mina (BIH Center for Regenerative Therapies (BCRT), Berlin-Brandenburg School for Regenerative Therapies (BSRT) at Charité )

poster

Introduction

Cellular senescence is an irreversible cell-cycle arrest program that has been associated with numerous biological processes. While emerging studies have extended its role to tissue repair, the focus has been on the effect of the senescence-associated secretory phenotype (SASP) rather than the direct role of the program beyond its paracrine effect. Using a previously...

433. Hypoxia reveals a new function of Foxn1 in the keratinocyte antioxidant defence system

Machcińska, Sylwia (Institute of Animal Reproduction and Food Research Polish Academy of Science)

poster

Introduction
The skin, as the outermost barrier of the body, is exposed to threats from the external environment including injuries, oxidative stress and UV irradiation. Therefore, efficient systems of repair and antioxidant protection of damaged skin that are achieved by an extraordinary mechanism involving the interaction of cells, cytokines and growth factors are vital for survival. In the...

438. Image-based evaluation of passage-induced senescence in human fibroblasts

Kimura, Kazue (Graduate School of Pharmaceutical Sciences, Nagoya University )

poster

[Introduction] Fibroblasts are the major cells that consist of connective tissues in animal tissues and are known as the main extracellular matrix (ECM) production cells to maintain the tissue physical properties. Although they are cells that are famous and frequently found in our body, their biological mechanisms, especially the differences from other similar fibroblastic cells are still...

431. Losartan is a potential modulator of hypoxia and articular chondrocyte hypetrophy

Malinauskas, Mantas (Lithuanian University of Health Sciences)

poster

Purpose: To investigate the effect of Losartan on articular chondrocyte hypertrophy in a monolayer culture under chemically induced hypoxia with CoCl2.

Materials & Methods: The 2nd passage of human knee chondrocytes was used to determine the cell viability in response to the treatment with Losartan or CoCl2 in the following concentrations 10-4 – 10-12 M. Chondrocytes were seeded in a...

432. Study of different components for efficient cryopreservation of Mesenchymal stem cells

Deryabina, Olena (State Institue of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine)

poster

INTRODUCTION: The question of efficient preservation of cells, especially Mesenchymal stem cells (MSC) becomes more and more urgent due to the impetuous development of cell therapy in clinics. There are two main problems in the improvement of mixtures used for cells cryopreservation. First is the application of DMSO as cryoprotectant, which is known to cause different side effects, and the...

435. The spatial and temporal relationship between cellular senescence and the process of skin healing

Valdivieso, Karla (Ludwig Boltzmann Research Group SHoW - Senescence and Healing of Wounds )

poster

The term "cellular senescence" refers to an irreversible cell cycle arrest that can be caused by a variety of stressors and is accompanied by an accumulation of damage, changes in cell shape and secretory profile, and epigenetic abnormalities in primary cells. There is an accumulation of senescent cells in development, aging and tissue regeneration; all of which are conditions with increased...