Conveners
S65-2 Vascularization for Tissue Engineering and Regenerative Medicine
- Arnaud Scherberich (Department of Biomedicine, University of Basel)
"Angiogenesis, the process by which new blood vessels sprout from existing surrounding ones, is essential for the survival of implanted tissue engineered constructs. In fact, the lack of proper re-vascularization and core necrosis is the main pitfall of many developed biomaterials (1). In this study, we describe a highly tunable hydrogel system for the growth of capillaries based on...
"Introduction
Fibrin biomaterial is widely used in the clinic as a tissue sealant and in pre-clinical research as a carrier material for growth factor delivery. In these applications, premature fibrin degradation leads to suboptimal tissue adhesion, recurrent bleeding and limited regenerative efficacy. Therefore, fibrinolytic inhibitors are commonly added into fibrin formulations, such as the...
"Immunohistochemistry and immunofluorescence for vascular network analysis play a fundamental role in basic science, translational research, and clinical practice. Due to their versatility and specificity, these techniques are also widely used in the tissue engineering context, where the study of neovasculature is crucial to assess the function of artificial tissue surrogates. However,...
"Introduction: Inducing axial vascularisation of tissue engineering constructs is a well-established method to allow an adequate support of large 3-dimensional tissues. Progenitor cell chemotaxis towards axially vascularized tissues and its role in inducing neo-vascularisation and tissue regeneration has not been well characterized.
Methodology: In a prospective randomized controlled study...
"Introduction: Neo-angiogenesis describes the development of new blood vessels and takes place during bone healing in the fracture gap. Angiogenesis and the establishment of a functional vascular network is closely associated with bone formation and critical for scar-free healing. Type H vascular endothelial cells (ECs), characterized by high expression of CD31 and endomucin (Emcn) have been...
"Introduction
Tissue engineering of human blood vessels is pursued as a clinical revascularization therapy as well as to develop in vitro human blood vessel models with native-vessel like organization. Clinical revascularization strategies that use autologous vessels are hampered by poor quality and limited availability. Synthetic vascular scaffolds may be used as alternative, but for...
"Introduction: Endochondral ossification (EO) is the process of bone development via a cartilage template. It involves multiple stages, including chondrogenesis, mineralization and angiogenesis. Importantly, how angiogenesis contributes to EO is not fully understood. To characterise the interaction between human mesenchymal stromal cells(hMSCs)-derived cartilage and blood vessels, we designed...
"Introduction
Prevascularization of tissue-engineered constructs before implantation is used to accelerate anastomosis with the host vasculature and thus to enhance successful implantation. To induce prevascularization, the co-culture of tissue-specific cells with endothelial cells is explored. To date, most co-culture studies are still conducted with human umbilical vein endothelial cells...
"INTRODUCTION: Mesenchymal stromal cells (MSCs) are potential candidates in tissue engineering applications. Nevertheless, MSC-based therapies have fallen short of the initial promise and hype due to the low MSC retention rate caused by the disruption of nutrient and oxygen supplies. Our previous studies established that the lack of glucose (but not oxygen) is fatal to human MSCs (hMSCs)...
