Speaker
Description
Bioprinting with high cell-density bioinks holds great promise for cellular condensation-based tissue engineering and regenerative medicine. However, achieving precise control over complex tissue structures and organization using high cell-density bioinks remains a significant challenge. Here, we introduce a novel approach for fabricating tissue-specific constructs by directly assembling high cell-density bioinks via three-dimensional printing into a photocrosslinkable and biodegradable hydrogel microparticle supporting bath. Three types of tissue-specific high cell-density bioinks were developed using either individual stem cells or stem cell aggregates, incorporating growth factor-loaded gelatin microparticles to guide differentiation. Once bioprinted into the photocrosslinked microgel bath, the bioinks undergo cellular condensation and differentiate along tissue-specific lineages, forming multiphasic structures such as osteochondral tissues. By varying the incorporated growth factors and cell types, this platform enables the engineering of diverse functional tissues with precisely controlled cellular architecture and organization. Furthermore, by printing patterned, cell-only prevascular bioinks, we demonstrate the formation of complex prevascular networks. These networks, combined with osteogenic microgels in the supporting bath, enhance the development of bone-like tissues.
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