Speaker
Description
Skeletal muscle tissue engineering is emerging as a cornerstone in the development of in vitro models, offering unprecedented opportunities to study muscle physiology and pathology. This work aim to develop high-throughput, custom-designed system for fabricating skeletal muscle constructs at a centimetre scale, addressing challenges in scalability, reproducibility, and biological relevance.
Our approach combines innovative biofabrication workflows with advanced imaging. A key feature of the system is its ability to screen and optimize multiple experimental parameters, such as biomaterial selection, cell concentration, mechanical stimuli, and in vitro culturing conditions, thanks to a simple and reproducible methodology. Novel metrics, such as the myo-index, provide quantitative benchmarks for comparing the architecture and maturation of artificial constructs to native tissues.
Furthermore, this versatile system provides a robust platform for analyzing biological processes critical to skeletal muscle development and maturation. Preliminary results highlight the potential of this approach to generate aligned and functional muscle tissue, offering insights into the interplay between C2C12 cellular dynamics and physical stimuli.
Our findings establish a transformative framework, providing scalable tools that bridge the gap between in vitro experimentation and in vivo functionality. This platform offers an easy-to-manage and reproducible in vitro model, opening new avenues for studying biological dynamics, cellular interplay, and developing therapeutic strategies for muscle-related disorders.
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