Speaker
Description
The advancement of disease modeling and drug testing has been consistently limited by the shortcomings of conventional in vitro and in vivo models, which often fail to accurately replicate the complex microenvironment of human tissues. To address this issue, our study introduces an innovative in vivo-mimicking three-dimensional human colon model that reproduces the structural, mechanical, physiological, chemical, and biological characteristics of native colon tissue more effectively than previously reported models. Our approach has led to the development of a dual-layered construct that replicates the intricate architecture of the colon, including its 3D luminal curvature and spontaneously self-organized crypt-like domains. This design enables the formation of crypt-like structures within a surrounding mucosal architecture, closely mirroring the colon’s native tissue composition, layered organization, and 3D topography. As a result, this in vivo-mimicking model provides a highly physiologically relevant platform for investigating colorectal cancer mechanisms and assessing therapeutic responses. By incorporating this model into our research, we have conducted extensive drug efficacy studies, particularly focusing on colorectal cancer treatments. Our findings shows the superior performance of this in vivo-mimicking model in drug response assessments, demonstrating its significant advantages over traditional 2D cultures and other existing 3D constructs.
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