Speaker
Description
Creating scalable and functional tissue constructs is a critical challenge in tissue engineering and cultured meat production. However, current methodologies are limited by inadequate nutrient delivery, resulting in inconsistent tissue growth and limited scale-up capability.
To address this, we developed a novel hollow fiber bioreactor (HFB) featuring densely packed arrays of semipermeable hollow fibers, serving as artificial circulation systems to uniformly distribute nutrients and oxygen throughout engineered tissues. Microfabricated anchors ensured precise fiber alignment, optimizing nutrient perfusion.
We demonstrated this approach through the biofabrication of centimeter-scale chicken muscle tissue, which exhibited enhanced sarcomere formation, improved texture, and elevated marker protein expression under active perfusion conditions. Robotic-assisted fiber threading significantly increased manufacturing efficiency, demonstrating potential scalability by assembling a bioreactor comprising over 1,000 hollow fibers for the production of large tissue constructs exceeding 10 grams.
This advancement not only enhances the quality and viability of cultured meat but also provides a robust platform for tissue engineering, potentially revolutionizing the mass production of complex, functional tissues and organs.
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