Speaker
Description
The development of three-dimensional (3D) cell-only tubular tissue constructs at small lumen sizes is a significant challenge in tissue engineering. This study presents an innovative strategy for fabricating multi-layered living conduits with defined geometries by 3D bioprinting multiple cell-only bioinks along with an oxidized and methacrylated alginate (OMA) microgel ink into a supporting OMA bath using setups of multiaxial nozzles. Single-layered and bi-layered cell-condensation conduits have been fabricated. Single-layered conduits were engineered using a coaxial nozzle with an OMA microgel ink and a cell-only bioink, while bi-layered conduits were produced using a triaxial nozzle with an OMA microgel ink and two different cell-only bioinks. These inks were simultaneously printed via a multiaxial nozzle into an OMA macromer support bath followed by photocrosslinking to stabilize the printed structures. Under in vitro culture, biomaterial-free cell-condensation layers were obtained by removing the OMA microgels and differentiated in a cocktail medium. The differentiated bi-layered tubular tissue constructs were subcutaneously implanted into mice, the bi-layered structures were well-retained over time and integrated with the host tissue. This study demonstrates a promising strategy for fabricating single-layered and bi-layered 3D living hollow cell condensations with controlled architectures through a combination of multiaxial extrusion and embedded bioprinting techniques. The technique has the potential to advance the development of engineered living tubular tissues with improved functionality and integration for future grafting applications.
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