Speaker
Description
Bone is a dynamic tissue composed of osteoblasts, osteoclasts, and osteocytes, which continuously undergo bone formation and resorption through a process known as bone remodeling. This balance between formation and resorption is tightly regulated by intricate cell–cell interactions. Bone organoid research aims to replicate the characteristics of native bone tissue in vitro; however, the development of bone organoids remains in its early stages due to the bone’s complex hierarchical architecture and the multifaceted interactions among its cellular components. In this study, an osteon-mimic three-dimensional (3D) structure was developed to emulate the structural and chemical features of natural osteons. To recreate the cellular microenvironment, a triple co-culture system comprising osteoblasts, osteoclasts, and osteocytes was established, enabling direct cell-to-cell communication. The osteon-mimic 3D structure was fabricated by coating a calcium/phosphate (Ca/P) composite onto a polycaprolactone (PCL) nanofiber membrane, effectively reproducing the lamellar organization of osteons. Morphological and chemical analyses confirmed the resemblance between the fabricated structure and native osteons. Furthermore, by optimizing the triple co-culture conditions, the differentiation and interactions among osteoblasts, osteoclasts, and osteocytes were successfully promoted. As a result, bone organoids replicating the morphological, chemical, and biological characteristics of natural osteons were successfully developed using the osteon-mimic 3D structure combined with triple co-culture.
32028911977