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Introduction:. An innovative approach combining pre-crosslinked methacrylated hyaluronic acid (HAPrime) with hybrid recombinant proteins was developed to create highly effective dressings for deep wound treatment. Treating deep wounds in animals, particularly pigs, remains a significant challenge due to risk of infection and delayed tissue regeneration. Emerging regenerative strategies, such as bioprinting wound dressings with bioactive components, offer promising solutions. Methacrylated hyaluronic acid (HAMA), a key component of the extracellular matrix, plays a crucial role in wound healing by supporting angiogenesis, tissue regeneration, and reducing inflammation. The bioprinting of HAMA-based dressings enables precise structural tailoring to the wound site, potentially enhancing the healing process. While this study was conducted in a porcine model due to its close resemblance to human skin, the developed strategy holds strong translational potential for future clinical applications in the treatment of complex or chronic wounds in humans.
This study evaluates the therapeutic efficacy of bioprinted HAMA-based dressings with recombinant protein in promoting deep wound healing in pigs, compared to traditional treatment methods.
Methods: The study involved experiments on a group of pigs, to whom bioprinted dressings made of hybrid materials (HAPrime and recombinant protein) were applied on wounds, compared with traditional dressings. Wound healing was evaluated by measuring wound closure and contraction, supported by histological and immunohistochemical analyses of tissue regeneration and inflammation. Gene expression profiling of inflammatory markers was performed, alongside assessment of the dressings antibacterial activity and monitoring of blood biochemical and morphological parameters over the course of the experiment.
Results: Preliminary results suggest that bioprinted dressings may effectively accelerate wound healing and enhance tissue regeneration compared to traditional treatment methods. The obtained outcomes clearly confirmed the utility of the developed approach—treated wounds demonstrated high healing potential, with no observable exudate and overall maintenance of a clean and stable wound environment. Additionally, the use of bioprinting technology enabled precise dressing conformation to wound geometry, ensuring personalized fit and optimal tissue contact. This likely contributed to improved healing dynamics and supports the clinical relevance of the strategy.
Discussion: The application of bioprinted dressings based on methacrylated hyaluronic acid and recombinant protein demonstrates potential for enhancing the healing of deep wounds in pigs. Improvements in wound closure rates, tissue architecture, and control of infection suggest that this approach could outperform traditional wound care methods. Further investigation will be needed to confirm the long-term benefits and to optimize the material properties for broader clinical application.
References:
Price RD, Myers S, Leigh IM, Navsaria HA. The role of hyaluronic acid in wound healing: assessment of clinical evidence. Am J Clin Dermatol. 2005;6(6):393-402. doi: 10.2165/00128071-200506060-00006. PMID: 16343027.
Neuman MG, Nanau RM, Oruña-Sanchez L, Coto G. Hyaluronic acid and wound healing. J Pharm Pharm Sci. 2015;18(1):53-60. doi: 10.18433/j3k89d. PMID: 25877441.
Fang H, Xu J, Ma H, Liu J, Xing E, Cheng YY, Wang H, Nie Y, Pan B, Song K. Functional materials of 3D bioprinting for wound dressings and skin tissue engineering applications: A review. Int J Bioprint. 2023 Mar 18;9(5):757. PMID: 37457938; PMCID: PMC10339425.
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