Speaker
Description
3D multicellular models provide valuable platforms for tissue regeneration, disease modeling, and biomaterial evaluation. However, the recreation of complex stratified systems that combine different tissue types and pathophysiological conditions remains challenging. In this work, we developed a multilayer construct integrating skin, muscle, and bone components using digital light processing (DLP) bioprinting. The bioinks were based on 5% (w/v) gelatin methacrylate (GelMA), with three different cell types: HFF-2 (skin layer), H9c2 (muscle layer), and SaOS-2 (bone/tumor layer) cells. The skin layer was supplemented with extracellular matrix (ECM) derived from dermal tissue for a more realistic environment. The printability, structural integrity, and cell viability of the constructs were evaluated after bioprinting and during culture. Fluorescence microscopy confirmed the successful spatial localization of each cell type within the respective layers, with high viability maintained up to five days. This tri-layer DLP-bioprinted model demonstrates the feasibility of fabricating complex multicellular constructs that not only mimic stratified tissues but also allow the integration of tumor cells, making it a promising platform for cancer research, drug testing, and as an alternative to animal models in preclinical studies.
