Speaker
Description
Constructing an in vitro vascularized liver tissue model that mimics the human liver plays a key role in promoting cell growth and biomimetic physiological heterogeneous structures and cellular microenvironments. However, the layer-by-layer printing method is greatly limited by the rheological properties of the bioink, making it difficult to form complex three-dimensional vascular structures in low-viscosity soft materials. To overcome this problem, in this study, we mixed low-viscosity biomaterials with gelatin microgels to form a cross-linkable biphasic embedding medium. This medium has the yield stress and self-healing properties, which is conducive to the efficient and continuous three-dimensional forming capability of the sacrificial ink. We controlled the filaments diameter by controlling the printing speed to adjust it from 250μm to 1000μm, and can accurately control the ink deposition position and filaments shape. We used in situ endothelialization to construct complex vascular structures and achieve close adhesion between hepatocytes and endothelial cells. In vitro study results showed that vascularized liver tissue model showed higher MRP2, albumin synthesis function, and higher enzyme activity than the mixed liver tissue. In summary, this method can quickly construct three-dimensional vascular structures in low-viscosity materials, and the resulting vascularized liver tissue model has good biological functions, opening up new opportunities for clinical applications.
74734123455
