Conveners
S03 4D bioprinting
- Shulamit Levenberg (Technion)
- Jeroen Rouwkema (University of Twente)
The field of cardiac tissue engineering is advancing rapidly toward the creation of functional, patient-specific therapies and whole-organ replacement strategies. In this talk, I will present recent progress in developing personalized biomaterials and leveraging state-of-the-art additive manufacturing technologies, including 3D and 4D printing, to fabricate vascularized cardiac tissues and...
The most widespread 3D bioprinting technologies are based on computer-controlled deposition of cells or assembly of cellular units, and thus cannot achieve spatial resolution better than few tens of micrometers. Lithography-based methods approach the problem from a different direction, by producing 3D structures within cell-containing materials and can therefore overcome this limitation. Among...
Introduction
Tissues and organs are composed of cells embedded in an instructive 3D extracellular matrix (ECM). Changes in mechanical properties of the ECM act as dynamic cues that guide cells through different stages of development. In synthetic 2D culture systems, matrix stiffness, viscoelasticity, and their variation have been shown to influence cell spreading and differentiation by...
4D bioprinting enables the fabrication of dynamic, adaptive materials capable of autonomous shape transformations in response to environmental cues. While most hydrogel-based actuators rely on external control, developing self-regulating soft robotic systems that operate autonomously in physiological conditions remains a significant challenge.
Here, we present 4D-printed protein-driven...
Introduction
Polymer-based hydrogels serve as excellent mimics of the extracellular matrix, enabling the generation of 3D in vitro tissue models.1 To improve the ability of these models to replicate tissue in vivo, there is great interest in enhancing model complexity.2 Towards this goal, the utilization of photoresponsive chemistries (e.g. polymerization/degradation) permits precise...
Introduction
The development of smart materials for bioprinting unlocks flexibility and control over the final construct characteristics and composition.1 Dynamic crosslinking allows the development of stimuli-responsive inks with intrinsic reversibility triggered by tunable physical and chemical conditions.2 Typically made of densely packed or jammed microgels, granular hydrogels offer...
Introduction
3D tissue printing has advanced significantly and can now create controlled vascular networks in engineered tissues for effective oxygen and nutrient transfer. However, a major challenge with cell-containing bioink hydrogels is their limited printing resolution, which affects the creation of small-scale features like capillaries. Here, we present a novel...
Introduction :
Volumetric bioprinting (VBP), enables the fabrication of complex cell laden architectures at high printing speeds in a layer-less fashion. Recently, this technology was refined to precisely pattern (bio)active molecules inside (bio)printed constructs post-fabrication, introducing possibilities for 4D printing. [1] A key challenge in biofabrication lies in replicating the...
