2025 International Conference on Biofabrication

Session

S09 Biofabrication of Anisotropy

15 Sept 2025, 13:30

Goethe

Conveners

S09 Biofabrication of Anisotropy

• Marcy Zenobi-Wong (ETH Zurich, Switzerland)
• Liliang Ouyang (Tsinghua University)

18. Harnessing chaotic bioprinting to engineer anisotropic microarchitectures for aligned tissue constructs and prevascularized models

Grissel Trujillo de Santiago (Tecnologico de Monterrey)

15/09/2025, 13:30

invited lecture

Anisotropy is a defining feature of many biological tissues, from skeletal muscle to vascular networks, and replicating this structural organization is crucial in the design of functional tissue constructs and relevant in vitro models. However, current biofabrication strategies often struggle to produce aligned architectures in a scalable, high-throughput, and versatile manner. Here, we...

17. Regulating Cellular Behavior in Hierarchical Tissue Engineering

Qi Gu (Institute of Zoology, Chinese Academy of Science | Beijing Institute of Stem Cell and Regenerative Medicine)

15/09/2025, 13:50

invited lecture

The engineering of hierarchical tissues with controlled cellular behavior is essential for replicating the complexity and functionality of natural organs. In our approach, we employed nanoengineered scaffolds to creat hybrid bioink or controlled macromolecule assembly in pre-crosslinked bioinks (yet not chemically crosslinked) to guide (stem) cell migration and promote anisotropic cellular...

269. Electrofibrillated Nanofibrillar Microbundles as Scaffolds for 3D Muscle Tissue Biofabrication

Taufiq Ahmad (Julius-Maximilians-Universität Würzburg)

15/09/2025, 14:10

podium presentation

Recreating the highly aligned and hierarchical structure of native extracellular matrix (ECM) remains a pivotal challenge in musculoskeletal tissue engineering, particularly for skeletal muscle, where anisotropic architecture is critical for function. In this study, we present a novel strategy that integrates melt electrofibrillation and 3D cell spheroid bioassembly to fabricate structurally...

456. Fibrous bioinks for the bioprinting of anisotropic scaffolds with micro- and nano-scale organization for in vitro skeletal muscle engineering

Gerardina Ruocco (Politecnico di Torino)

15/09/2025, 14:20

podium presentation

Introduction
The functionality of skeletal muscle tissue (SMT) hinges on its hierarchical anisotropic microstructure. Conventional 2D cell cultures are poorly biomimetic and unable to properly support in vitro engineering of SMT, triggering the research towards more reliable and predictive in vitro models [1]. Biomimetic fiber-based scaffolds provide topographical cues supporting SMT in vitro...

362. Continuous and Layerless 3D Printing of Aligned Tissues

Jakub Janiak (ETHZ)

15/09/2025, 14:30

podium presentation

Introduction:
Biofabrication of highly aligned 3D tissues like nerves, tendons, or muscles remains challenging due to insufficient scaffold cues to guide cell alignment, proliferation, extracellular matrix secretion, and maturation into functional tissues. Previously, we showed the ability to create such tissue constructs using filamented light (FLight) biofabrication approach1. We expanded...

363. Advancing Anisotropy in Engineered Skeletal Muscle via Bioactive Porous Hydrgel Interface

Nehar Celikkin (Institute of Physical Chemistry Polish Academy of Sciences)

15/09/2025, 14:40

podium presentation

Skeletal muscle exhibits a highly organized, anisotropic architecture, where fascicles - bundles of aligned myofibers - are embedded within a connective tissue matrix. Such structural organization is the key for skeletal muscle achieving uniaxial contractions, mechanical stability, and functional performance in vivo.
The presented engineered skeletal muscle (eSM) platform aims the development...

451. Hierarchical Structures Biofabricated from Ultrathin Aligned Collagen Membranes Provide Physical Barriers for Molecular Transport

Axel Guenther (University of Toronto)

15/09/2025, 14:50

podium presentation

Barrier tissues in the body often form tubular structures that regulate molecular and water transport to maintain physiological function. We present a hierarchical biofabrication strategy to engineer such structures with tailored barrier properties, using ultrathin, wet-spun collagen membranes exhibiting high fibrillar alignment, compaction, and a Darcy permeability of 3.84 × 10⁻¹⁶ m². By...