Conveners
S09 Biofabrication of Anisotropy
- Marcy Zenobi-Wong (ETH Zurich, Switzerland)
- Liliang Ouyang (Tsinghua University)
Anisotropy is a defining feature of many biological tissues, from skeletal muscle to vascular networks, and replicating this structural organization is crucial in the design of functional tissue constructs and relevant in vitro models. However, current biofabrication strategies often struggle to produce aligned architectures in a scalable, high-throughput, and versatile manner. Here, we...
The engineering of hierarchical tissues with controlled cellular behavior is essential for replicating the complexity and functionality of natural organs. In our approach, we employed nanoengineered scaffolds to creat hybrid bioink or controlled macromolecule assembly in pre-crosslinked bioinks (yet not chemically crosslinked) to guide (stem) cell migration and promote anisotropic cellular...
Recreating the highly aligned and hierarchical structure of native extracellular matrix (ECM) remains a pivotal challenge in musculoskeletal tissue engineering, particularly for skeletal muscle, where anisotropic architecture is critical for function. In this study, we present a novel strategy that integrates melt electrofibrillation and 3D cell spheroid bioassembly to fabricate structurally...
Introduction
The functionality of skeletal muscle tissue (SMT) hinges on its hierarchical anisotropic microstructure. Conventional 2D cell cultures are poorly biomimetic and unable to properly support in vitro engineering of SMT, triggering the research towards more reliable and predictive in vitro models [1]. Biomimetic fiber-based scaffolds provide topographical cues supporting SMT in vitro...
Introduction:
Biofabrication of highly aligned 3D tissues like nerves, tendons, or muscles remains challenging due to insufficient scaffold cues to guide cell alignment, proliferation, extracellular matrix secretion, and maturation into functional tissues. Previously, we showed the ability to create such tissue constructs using filamented light (FLight) biofabrication approach1. We expanded...
Skeletal muscle exhibits a highly organized, anisotropic architecture, where fascicles - bundles of aligned myofibers - are embedded within a connective tissue matrix. Such structural organization is the key for skeletal muscle achieving uniaxial contractions, mechanical stability, and functional performance in vivo.
The presented engineered skeletal muscle (eSM) platform aims the development...
Barrier tissues in the body often form tubular structures that regulate molecular and water transport to maintain physiological function. We present a hierarchical biofabrication strategy to engineer such structures with tailored barrier properties, using ultrathin, wet-spun collagen membranes exhibiting high fibrillar alignment, compaction, and a Darcy permeability of 3.84 × 10⁻¹⁶ m². By...
