Conveners
S13 Biofabrication strategies for engineering microarchitectural complexity
- Shrike Zhang (Harvard University)
- Khoon Lim (University of Sydney)
Bioprinting technology holds tremendous potential for developing artificial tissues and organs that mimic the complexity of their native counterparts. However, despite considerable progress in the field, bioprinted tissues are structurally and functionally immature compared to their native counterparts. This limits their effectiveness as implants for regenerative medicine or predictive...
Rapid in situ bioprinting on complex, human-scale anatomical surfaces remain a key challenge for clinical translation. Here, we present a gravity-independent, conformal bioprinting strategy using bi-phasic granular bioink and multinozzle printheads capable of adapting to arbitrary surface curvatures. The bioink comprised of jammed gelatin microgels suspended in a fibrinogen matrix exhibits...
Introduction
Despite significant developments in endothelial-cell (EC) manipulation techniques, a proper in vitro model of a functional microvasculature with controlled local interconnectivity under well-defined global architecture is still lacking. Here, we report the generation of such controlled multi-scale vascular networks via manipulation of tens of sprouting EC โseedsโ. We exploit...
Self-assembling peptide amphiphiles (PAs) offer a unique combination of biofunctionality, structural tunability, and nanofibre alignment under shear, making them highly promising materials for advanced in vitro tissue models. Despite this potential, their inherent fragility and the lack of scalable structuring strategies have restricted their wider adoption for in vitro modelling. To address...
Purpose
The primary role of articular cartilage (AC) is to provide frictionless joint movement while transferring loads to the underlying bone [1]. Thanks to its unique hierarchical arcade-like collagen type II fiber organization, AC withstands extreme mechanical forces. While numerous regenerative medicine approaches strive to replicate the native architecture of AC, none have achieved...
INTRODUCTION: The biological world is curved from the subcellular to the continental length scale [1]. Cells sense the complex shapes of their surroundings and respond to these stimuli through the transduction of physical stimuli into biochemical responses. In vitro, designed, cell-scale 2.5D curvatures have been shown to drive cell migration responses [2]. However, synthetic polymer...
Light-based biofabrication techniques like filamented light (FLight) offer robust architectural alignment but are often limited in compositional complexity[1]. Conversely, acoustic assembly enables non-contact, label-free patterning of cellular spheroids or bioactive particulates, yet lacks architectural guidance at the microstructural level[2]. To achieve tissue functionality, such as muscle,...
Introduction
Over the past two decades, robotic additive manufacturing (RAM) has been introduced into fields such as aerospace and construction, and multiple studies have shown the potential of RAM for Regenerative Medicine (RM). However, hardware limitations of current industrial robots reduce the print resolution of small volume RAM hindering the wider adoption into RM. Eye-in Hand (EH)...
