Conveners
S16 Bioprinting vasculature
- Riccardo Levato (University Medical Center Utrecht)
- Shulamit Levenberg (Technion)
Vascularization is critical for successful tissue regeneration, as it ensures adequate oxygen, nutrient delivery, and waste removal within engineered constructs. Without a functional vascular network, large or complex tissues are prone to necrosis and poor integration with host tissues. Rapid and stable vascular ingrowth is therefore essential for the survival and functionality of regenerated...
We are attempting to rebuild tissue-like structures from cells as an approach to better understand development, physiology and disease. Here I will describe our recent work in engineering perfusable vasculature to understand the complex interplay between architecture, forces, signaling, and cellular adhesions in regulating vessel formation, function, and dysfunction. The presentation will...
INTRODUCTION: Embedded bioprinting is a promising additive manufacturing technique permitting the fabrication of large-scale, freeform, complex 3D tissue constructs. During the development of new (bio)inks, the shape-stability of extruded strands plays a major role. To stabilize strands post-extrusion, while allowing for smooth extrusion with limited pressures, shear thinning inks are commonly...
While three dimensional-bioprinting has already gained global attention as a rapidly advancing field, the creation of properly vascularized tissues remains challenging. Extrusion-based bioprinting is specifically interesting for the fabrication of large tissues, but has a limited resolution. Therefore, with extrusion-based bioprinting, microvasculature formation through self-assembly is...
The fabrication of biomimetic, multiscale, and multimaterial vascular networks remains a central challenge in tissue engineering and organ fabrication. We introduce an advanced light-based bioprinting framework that combines three advanced methodologies - Holographic Optical Tweezers Bioprinting (HOTB), multimaterial Digital Light Processing (DLP), and volumetric DLP bioprinting - to overcome...
Introduction
Tissue engineering aims to develop functional tissues for regenerative medicine. To achieve this, vascularization is essential, particularly in thick constructs where diffusion alone is insufficient. Neural tissues are especially challenging due to their high metabolic needs and reliance on dense vascular networks1. Although recent strategies have attempted to recreate...
The development of physiologically relevant vascular models remains a major challenge in tissue engineering, particularly when using extrusion-based 3D bioprinting with soft, liquid-like biomaterials. To overcome limitations related to structural instability and gravity, the Freeform Reversible Embedding of Suspended Hydrogels (FRESH) method enables mid-air bioprinting within a temporary...
