Conveners
S18 Chaotic Printing: A New Bioprinting Paradigm
- David Dean (University of Wisconsin-Madison)
- Alicia Bonilla Vázquez (Universidade de São Carlos (IQSC-USP))
Biofabrication increasingly demands creative strategies to fabricate structured and functional constructs that can meet healthcare challenges. Chaotic printing and bioprinting offer a unique and accessible way to build internal complexity in materials, leveraging the deterministic behavior of laminar flows.
Our journey began with a miniaturized journal bearing system, where controlled chaotic...
In this work, we present our latest advances in the biofabrication of monoculture and polyculture tumor niches using chaotic bioprinting as an enabling strategy for in vitro cancer research.
We first focus on the fabrication of monoculture breast cancer models designed to study tumor cell migration and invasion. Understanding these processes requires sophisticated models that accurately...
Introduction
A substantial body of scientific evidence demonstrates that gut microbiota plays a central role in human health and disease1,2. Understanding bacteria–host interactions in the human gut is essential for advancing microbiome research. Early 3D culture systems relied on physical membranes to separate bacteria from the intestinal epithelium model, which often limited the ability to...
Introduction
Probiotic therapies offer great potential for addressing gut dysbiosis, but current approaches are limited by low strain diversity, high production costs, and the challenges of culturing strict anaerobes. Some promising approaches include co-culture techniques, but traditional methods have drawbacks, including nutrient competition and instability due to different growth rates...
Introduction:
Internal cellularization of thick tissue scaffolds remains a central challenge in tissue engineering, often requiring complex and costly technologies. In this study, we developed a cost-effective chaotic bioprinting strategy to fabricate compartmentalized hydrogel filaments that simultaneously provide physical cues (hollow microchannels for enhanced mass transport) and chemical...
Problem: A fundamental problem for most tissue engineered constructs is the inability to provide a fully perfusing blood supply on implantation. That slows, if not prevents, healing within the treated site. If possible, standard-of-care autologous bone grafts are transplanted with their native vascular pedicle. In these cases, healing is unlikely to be hindered by an insufficient blood supply....
Introduction.
The growing demand for sustainable meat alternatives has accelerated research on culture meat. However, scalable production of fully edible meat-like structured constructs remains a challenge. In this study, we use chaotic bioprinting, extrusive bioprinting enabled by chaotic advection, using an enzymatic crosslinking agent, transglutaminase (TGase), to fabricate...
