Conveners
S32 Post Bioprinting Process
- Denisa Kaลokovรก (Czech Technical University in Prague, Faculty of Biomedical Engineering)
- Koichi Nakayama (Saga University)
Bioprinting technologies, including those developed by our group, have already entered the stage of clinical application. However, most of these achievements remain in the Proof of Concept and/or phaseI/II, and many challenges must be overcome before bioprinting can become a widely adopted medical practice. One critical aspect is the series of steps that follow the industrial printing...
Introduction: Customized bioreactors can replicate diverse physiological conditions, such as shear stress, pulsatile pressure, and strain, while enhancing diffusion and nutrient exchange. These conditions stimulate cellular processes including proliferation, differentiation, gene expression, and substrate remodeling. However, in two-dimensional cultures, these stimuli promote adipose-derived...
3D bioprinting has emerged as a promising technology in tissue engineering and regenerative medicine. Collagen, given its natural abundance in the extracellular matrix and excellent biocompatibility, serves as an ideal biomaterial for the preparation of printable bioinks. This study explores the optimization of collagen bioink properties as well as cultivation strategies and microvascular...
INTRODUCTION
Advanced in-vitro systems are fundamental to investigate cell dynamics and crosstalk occurring within the same or different districts of the human body.
In the case of chronic lymphocytic leukemia (CLL), given the importance of the microenvironment, there is a strong need for new tools to unveil key pathogenetic mechanisms and perform more reliable drug testing ex-vivo. Little...
Perfusion platforms are increasingly used to replicate in vivo vascular environments and to investigate how the interplay between vascular geometry, surface properties, and flow dynamics influences the physiology of endothelial cells lining the lumen of vasculature. Here, we present an approach that combines 3D printing, soft lithography, and advanced surface modification to create perfused...
Biofabrication has revolutionized the way we design in vitro models of human physiology. Advances in 3D bioprinting, scaffold engineering, and stem cell biology have enabled the creation of increasingly sophisticated tissues. Biofabrication permits spatiotemporal control over cell-cell and cell-extracellular matrix communication and thus the recreation of tissue-like structures [1,2]. Yet,...
Extrusion-based bioprinting enables fabrication of living constructs with tissue-like features but poses significant challenges for maintaining post-printing cell viability due to complex mechanical stresses. In this study, we leverage the integrated in-line rheological modules of the RevoBITs Byte 1 bioprinter to directly quantify shear and elongational stresses during printing and correlate...
