Conveners
Poster Session I: Poster Exhibition
- Carmelo De Maria (University of Pisa)
- Marco Costantini (Institute of Physical Chemistry Polish Academy of Sciences)
Poster Session I: Poster Exhibition
- Marco Costantini (Institute of Physical Chemistry Polish Academy of Sciences)
- Carmelo De Maria (University of Pisa)
Description
posters are on display whole day on Sunday and Monday
INTRODUCTION
The skin has restricted regeneration potential in the presence of certain illnesses. In certain
circumstances, the using of specific treatments is strongly recommended in order to improve the
wound healing process. Polysaccharide-based biomaterials exhibit a high potential for wound healing
because this category maintains wound hydration, allows gas exchange and absorbs...
The dermal-epidermal junction is critical in maintaining skin homeostasis, providing mechanical support, and facilitating nutrient exchange. One of the key features of this interface is the papillary dermis, which is characterized by a series of undulations that increase surface area for diffusion between dermal and epidermal compartments. During aging, these undulations flatten, resulting in...
Introduction
In Europe, around 60 million people have diabetes, and approximately 15% will develop diabetic foot ulcers (DFUs), with annual treatment costs of โฌ4โ6 billion [1]. Neuropathy hinders early wound detection, complicating DFU management [2]. Electrospun structures mimic the skinโs extracellular matrix but their 2D nature limits full regeneration [3]. This study proposes to create a...
Introduction: The development of biofabrication requires reliable and standardized methods for quantifying a wide range of printing techniques and tissue models to ensure a successful translation into medical applications. With the rise of convergence and the integration of multiple materials, printing processes are becoming increasingly complex, posing challenges for structural analysis....
Cartilage injuries remain a major clinical challenge due to the tissueโs limited self-healing capacity. Standard treatments, such as microfracture or autologous chondrocyte implantation, are often insufficient in restoring full function and structure of damaged cartilage. In this study, we present the SmartPiezo scaffold - a novel 3D printed, bio-based, and biodegradable construct designed...
Introduction
Large bone defects caused by trauma or surgical resection often require scaffolds that both promote regeneration and prevent bacterial infection. Chitosanโagarose (CA) hydrogels are biocompatible and printable, but their limited mechanical strength and weak antibacterial capacity remain challenges. Incorporating metal oxide nanoparticles (NPs) such as ZnO, MgO, and CaO offers a...
Introduction
Osteochondral defects involve damage to both the articular cartilage and the underlying subchondral bone, often resulting in joint instability and risk of osteoarthritic degeneration [1]. Recent research in treating such complex joint lesions focuses on developing 3D porous biomaterials, engineered with precise size and shape specifications through additive manufacturing...
Diabetes mellitus (DM) is a complex metabolic disease characterized by impaired glucose metabolism (hyperglycemia), leading to severe and long-term complications, such as kidney failure, stroke, peripheral neuropathy, nephropathy and, above all, foot ulcers.
In the last years, 2D in-vitro cell culture systems and 3D in-vivo animal models have been particularly useful in understanding the...
Successful bone regeneration in clinical research still remains a great challenge due to the complex morphology and the recent advances aim to enhance the rate of bone healing using biofabrication strategies such as 3D bioprinting loaded with growth factors. 3D bioprinting has tremendously boosted the transition from conventional regenerative procedures to customized patient-specific...
Introduction/Objectives
We have previously shown that an aqueous two-phase system (ATPS) composed of gelatin methacryloyl (GelMA) and dextran can form large interconnected pores via phase separation process [1]. As such open porous gels could be beneficial for 3D tissue engineering, we investigated whether the novel material could be processed by microextrusion and drop-on-demand printing...
Tissue engineering (TE) aims to regenerate damaged or diseased tissues by replicating their native structure, composition, and function. This goal is particularly challenging in the context of musculoskeletal tissuesโsuch as articular cartilage, meniscus, ligaments, and tendonsโwhose unique biomechanical functions are tightly linked to their extracellular matrix (ECM) architecture. Damage to...
A Modular Endoscopic Projection System for Spatially Patterned Photocrosslinking in Cartilage Repair
Theofanis Stampoultzis1, Parth Chansoria1, Marco Raffo2, Amedeo Franco Bonatti2, Giovanni Vozzi2,3, Marcy Zenobi-Wong1*
1Tissue Engineering and Biofabrication Lab, ETH Zurich
2Research Center "E. Piaggio", University of Pisa, Pisa, Italy
3Department of Information Engineering, University of...
Introduction
Breast cancer continues to be one of the leading causes of cancer-related mortality among women worldwide [1,2]. Conventional 2D cultures and animal models fall short in accurately replicating the breast tumor microenvironment, often lacking translational relevance [3]. The development of three-dimensional (3D) in vitro models through hydrogel-based bioprinting offers a promising...
Type 1 diabetes mellitus (T1D) is a chronic autoimmune disease that leads to hyperglycemia due to the loss of pancreatic ฮฒ cells. One of the most effective methods to treat T1D is pancreatic islet transplantation. However, transplanted islets lose their function because of immune reactions. To prevent immune-mediated damage, semipermeable encapsulation strategies are commonly employed....
Pediatric bone tissue engineering presents distinct challenges related to the growing patient including: the need for a construct strategy that preserves growth plates; bone generation that remodels in parallel with skeletal development to prevent long-term growth restrictions; and a degradation profile that aligns with the process of bone generation. Despite the recognized limitations of...
Introduction
Cartilage defects pose significant challenges in terms of healing. Current treatments have limitations in size, availability, or durability.[1,2] Biofabrication aims to restore tissue functionality by placing biological active components in a pre-defined 3D organization, typically using soft hydrogels for cell preferences.[3] These hydrogels can be mechanically reinforced with...
Bone defects in the oral and maxillofacial region, often resulting from trauma, congenital anomalies, degenerative conditions, or therapeutic procedures, present significant clinical challenges. Guided bone regeneration (GBR) and guided tissue regeneration (GTR) are widely adopted strategies that rely on barrier membranes to spatially direct tissue growth. However, conventional collagen-based...
Background: The development of functional tracheal tissue requires both mechanical stability and cellular functionality, yet creating biomimetic tracheal structures remains a significant challenge. In this study, we developed tissue-specific hydrogels derived from decellularized extracellular matrices (ECM) of cartilage, submucosa, and muscle using proprietary methods. In tissue engineering,...
Introduction
Osteoarthritis (OA) is a degenerative disease affecting osteochondral (OC) tissue, leading to pain and joint dysfunction. Current treatments are often limited by availability, efficacy, and cost, highlighting the need for innovative therapeutic approaches. To address this challenge, we propose a novel tool EndoFLight, an advanced in situ 3D bioprinting platform designed for...
Introduction: The osteochondral unit composed of subchondral bone, calcified cartilage and hyaline cartilage fulfills a crucial function in joint homeostasis and load transduction. During osteoarthritis, this unit is severely damaged resulting in impaired joint function. Strategies to regain function range from autologous chondrocyte implantation to tissue engineered cartilage to osteochondral...
Peripheral artery disease is the third leading cause for morbidity worldwide, demanding a multimodal therapy for more than 200 million people. Current therapeutic options for more severe cases range from medication therapy to surgical interventions. Synthetic vascular bypass grafts are the current gold standard in the surgical treatment of patients with no suitable autologous graft available....
Periodontal disease(PD) is a chronic infection caused by gram-negative bacteria, with a high prevalence worldwide. It is considered the second leading cause of dental problems.The disease begins with an imbalance between the oral microbiota and the hostโs immune system, triggering vascular changes and inflammation.Common symptoms include bleeding and swollen gums, which compromise tissue...
Introduction
The standardizability in fabrication of any biological tissues in high numbers is a major promise of the field of biofabrication. The acceptance of biofabrication methods in the industry continues to be hampered by the fact that bioinks are assembled in non-physiological structures (especially for soft tissues) combined with an absent application-orientated model design.
With...
Three-dimensional (3D) cellular models to study various kind of diseases mimic a more in <span style="box-sizing:border-box; margin:0px; padding:0px">vivo-like</span> native environment compared to 2D cellular models. Our study focuses on breast cancer, which represents the second most common cancer to metastasize to the brain. Triple-negative breast cancer, HER2+, and Luminal B breast...
Despite significant advancements in 3D bioprinting, challenges remain in achieving high printing speed, efficiency, and homogeneity in biological samples. These limitations constrain the practical application of bioprinting in tissue engineering, particularly in the fabrication of complex tissues like skeletal muscle. This project introduces a novel high-throughput combina torial...
Introduction: Corneal transplantation is the gold standard procedure to cure corneal diseases that can lead to blindness. Fabrication of corneal tissues is a promising solution to overcome the shortage of human donors. Our future goal is to utilize a bioprinter mounted to a robotic arm to print corneal tissue directly onto the eye of the patient. One interesting cell type for in situ and in...
Introduction
For decades, two-dimensional (2D) cell culture systems have been fundamental for studying cellular metabolism, differentiation, and drug efficacy. These simple models enable straightforward experimental design but does not replicate native tissue complexity. Important features such as physiological cell density, structural organization, and vascularization are absent. Recently,...
Expanding the Melt-Electro Fibrillation Polymer Library for Advanced Biofabrication Applications
Tamaki Kumauchi1, Kristina Andelovic1 and Jรผrgen Groll1
1Department of Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication (IFB), and Bavarian Polymer Institute (BPI), University of Wรผrzburg, 97070 Wรผrzburg, Germany
Introduction: Melt-Electro...
Introduction
Tissue decellularization is widely used in tissue engineering and regenerative medicine. Currently, most established decellularization methods use detergents, which have long decellularization processes with inconsistent results. Residual chemicals used in decellularization or changes in the biochemistry and structure of extracellular matrix (ECM) proteins may be responsible for...
Chitosan, a naturally derived polysaccharide, has gained significant attention in tissue engineering due to its excellent biocompatibility, biodegradability, and bioactivity. However, conventional chitosan sourced from crustacean shells poses limitations such as allergenicity and ethical concerns. In this study, we extracted and characterized mushroom-derived chitosan from Pleurotus ostreatus...
Conventional local drug delivery systems often fail to support sustained drug release, while many biomaterials used in tissue engineering and transplantation lack the necessary stability, biocompatibility, and capacity to support vascularization required for long-term graft survival and function. In order to overcome these restrictions, 3D bioprinting has become a potent technique that makes...
Introduction: The glomerular filtration barrier (GFB) is a highly specialized structure responsible for blood filtration in the kidney; is composed of podocytes, glomerular basement membrane and fenestrated endothelial cells. Dysfunction of this barrier is a hallmark of many glomerular diseases. However, the current systems in vitro cannot mimic efficiently the 3D organization and the...
Introduction
Two main technologies of Biofabrication are bioprinting and scaffold generation. [1] Bioprinting can be used with cells in the matrix, while scaffold generation is cell-free and cells are attached afterwards. Both have their distinct advantages, e.g. bioprinting enables the generation of complex tissue hierarchies in one step, while scaffolds can guide cell elongation via...
Introduction
Cartilage tissue regeneration has been significantly advanced through the development of artificial scaffolds, including three-dimensional (3D) electrospun structures. A key challenge in designing in vitro osteochondral models is creating scaffolds with a functional barrier that mimics the native tidemarkโseparating cartilage and boneโwhile still permitting cellular communication...
Development of patient specific composite scaffold using 3D printing for regeneration of craniofacial bone tissue
Monireh Kouhi1*, Mohammad Khodaei2, Saba Yousefi3
1. Dental Materials Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran,
2. Materials Engineering Group, Golpayegan College of Engineering, Isfahan...
Introduction:
Cancer is one of the leading causes of death worldwide and although developing new therapies can help fight this disease, cancers which origins from breast, lung or prostate can undergo metastasis process leading to development of secondary tumors in other parts of the human body [1]. Bone is the organ to which cancer often metastases and to pioneer cancer therapies, creation of...
Among the challenges associated with the regeneration of critical-sized bone defects, the most significant issue is the insufficient delivery of oxygen and nutrients, which impedes the optimal development of osteocytes. A potential solution lies in the use of biomaterials that can induce bone formation from hypertrophic chondrocytes cartilage tissue via endochondral ossification. For the...
Skeletal muscle tissue engineering is emerging as a cornerstone in the development of in vitro models, offering unprecedented opportunities to study muscle physiology and pathology. This work aim to develop high-throughput, custom-designed system for fabricating skeletal muscle constructs at a centimetre scale, addressing challenges in scalability, reproducibility, and biological...
Impact of cellular interactions on microarchitecture, matrix remodeling, and tenogenesis
in bioreactor stimulated 3D tendon model
Amrutha Datla, Subha Narayan Rath*
Regenerative Medicine and Stem cell Laboratory, Indian Institute of Technology Hyderabad, India - 502284
ABSTRACT:
Introduction: Tendon injuries are widespread, often leading to tendinopathy due to lack of
early recognition,...
Introduction
One of the most significant challenges in organ bioengineering is developing functional vascular networks. Proper vascularization is critical for transporting oxygen, nutrients, and signaling molecules, while also removing waste. In bionic organs, poor vessel formation limits nutrient exchange and cell migration, reducing transplant quality and long-term survival [1], [2]. The...
Introduction:
On-chip vascular microfluidic models provide powerful platforms for studying vasculature and its diseases in vitro. These models enable focused investigation of specific vascular layers, such as the endothelium, and the influence of hemodynamics on it. While traditional plastics or glass-based fabrication allows for defined microchannel architecture, its inherent stiffness and...
Considering the increasing incidence of bone-degenerative diseases and injuries, including osteoarthritis, especially in societies where aging is associated with increased obesity and poor physical activity, the repair of bone defects is one of the major challenges in medical science. Due to the difficulties, high cost, risks of surgery, severe allergic reactions, and ultimately rejection of...
Decellularized scaffolds are becoming important tools within tissue engineering. This work compares the effectiveness of three decellularization protocolsโSDS, HโOโ, and Triton X-100 for goat urinary bladder tissue on the preservation of ECM components and the functionality of scaffolds. The cellular removal and ECM preservation were assessed through histological staining with H&E,...
Introduction
Cell-seeded scaffolds made from natural or synthetic materials are widely tested in animal models as biodegradable implants for treatment of joint disease (1). One of the most commonly used material for scaffold production is polycaprolactone (PCL), known for its biocompatibility and mechanical properties close to human cartilage. To validate cell distribution within scaffold and...
Introducing Optical Fiber-Assisted Bioprinting (OFAB) as Novel 3D Bioprinting Method
Maximilian Pfeiffle, Alessandro Cianciosi, Tomasz Jรผngst
Department for Functional Materials in Medicine and Dentistry, University of Wรผrzburg, Pleicherwall 2, 97070 Wรผrzburg, Germany
Introduction: To accelerate scientific progress in tissue engineering and regenerative medicine, new accessible 3D...
Introduction: Nasal septum defects, whether resulting from infection, trauma, or prior surgery, remain difficult to repair due to shortcomings in existing implant materials [1]. Conventional implants often suffer from inadequate mechanical strength, rapid degradation, or insufficient bioactive cues for both cartilage and mucosal regeneration [1]. As a result, patients risk poor functional...
Osteoarthritis (OA) is one of the most prevalent musculoskeletal disorders globally, with its burden escalating due to the aging population and the absence of disease-modifying therapies 1. Despite its debilitating nature, current treatments remain palliative, focusing primarily on pain management or surgical intervention, with no curative options. The pathogenesis of OA involves a complex...
Introduction
Lipid nanoparticles (LNPs) represent a promising and versatile platform for the non-viral delivery of genetic material, including microRNA (miRNA), which plays a crucial regulatory role in gene expression. While LNPs have already demonstrated clinical success as mRNA carriers in vaccines, most notably in COVID-19 immunization strategies, their application in miRNA delivery opens...
INTRODUCTIOโฏ
The synovial lining is responsible to produce synovial fluid into the joint capsule. The synovial fluid acts as a lubricating and protecting layer for the articular cartilage to ensure pain-free frictionless movement of the articular joint. The synovial lining is prone to inflammation from injury, overuse or inflammatory arthritis (1), but modelling these events in vitro, while...
Introduction
Cartilage defects present significant challenges in long-term repair, with current treatments, such as cell-based therapies, often failing to restore sustained biomechanical function due to poor integration and/or lack of type II collagen organization[1]. The native depth-dependent fiber orientations in the arched collagen structure provide the mechanical support necessary to...
Corneal blindness is one of the leading causes of blindness worldwide. A cornea transplant surgery provides ultimate cure, however, it is restricted by donor tissue shortage. Thus, we aim to develop 3D bioprinted full thickness corneas to address this lack of donor material. To ensure robust transport, handling, and suturing, the bioprinted cornea's mechanical properties needs to be improved....
Introduction
Over the past few decades, extensive research has been actively conducted for the fabrication of human tissues to, amongst many applications, understand the effects of a wide range of chemicals on human health and the environment (1). Thus, the need for the development of innovative assessment tools that provide reliable results in identifying and regulating the risks of...
Introduction:
The low success rate of oncological drugs in clinical trials highlights the need for predictive preclinical models because the traditional 2D cultures and animal models fail to replicate human tumor microenvironments fully. The researchers have seen 3D bioprinting as a promising technology for the fabrication of more physiologically relevant tissue models. Bioinks for such...
Injectable Nanofibrous Microcarriers with Tunable PVA/Tannic Acid Ratios for Controlled miRNA Delivery in Intervertebral Disc Regeneration
Farzaneh Sabbagh, Paweล Nakielski
Institute of Fundamental Technological Research, Polish Academy of Sciences (IPPT PAN), Warsaw, Poland
Introduction: Degenerative disc disease (IDD) induces persistent back discomfort resulting from the degeneration...
Introduction: Reproducibility, scalability and adaptability are crucial aspects for any fabrication method that desires to address cell laden materials that shall be used for tissue engineering or drug screening approaches. Biomimetic fiber-based scaffolds are the most prominent among these as they mimic the fibrillar nature of many ECM proteins or other fibrous structures found therein, such...
Introduction
Injectable hydrogels with submicron dimensions that can provide both mechanical support and biological functionality to encapsulated cells are gaining attention for in vivo delivery of therapeutic cells and biomolecules [1,2]. In recent developments, hydrogel crosslinking triggered by visible light using the ruthenium(II) tris-bipyridyl complex (Ru) in conjunction with sodium...
Quercetin, a flavonoid known for its antioxidant properties, has recently garnered attention as a potential neuroprotective agent for treatment of the injured nervous system. The repair of peripheral nerve injuries hinges on the proliferation and migration of Schwann cells, which play a crucial role in supporting axonal growth and myelination. In this study we synthesized Quercetin-derived...
Introduction: Advancements in biofabrication are poised to revolutionize healthcare by providing innovative solutions to complex medical challenges. A recent area of excitement is the integration of the extracellular matrix (ECM), which plays a pivotal role in tissue homeostasis, cellular signalling, and regenerative processes, into biofabrication approaches, making it the ECM a critical...
Introduction
Natural biomaterials have opened promising avenues in bioprinting and tissue engineering by providing native-like environments for regenerative therapies. These materials offer significant biological advantages but also introduce challenges related to variability, immunogenicity, and contamination risks [1]. The regulatory framework is gradually evolving to address these...
The meniscus plays a critical role in load transmission, shock absorption, and joint stability within the knee. However, its limited regenerative capacity, particularly in the inner avascular zone, contributes to the progression of degenerative joint diseases following injury. The complex zonal organization of the meniscusโcharacterized by a fibrous outer zone rich in type I collagen and a...
Decellularized human chorionic membrane (HCM) offers a promising biomaterial for tissue engineering due to its rich extracellular matrix (ECM), inherent biocompatibility, and accessibility. This study compares the efficacy of different decellularization methods in generating acellular HCM scaffolds while preserving structural and functional ECM integrity. Cellular removal and ECM preservation...
Introduction Significant efforts have concentrated on creating various synthetic and natural biomaterials that mimic the native bone extracellular matrix, promote osteogenic differentiation, and improve effective bone regeneration. 3D printing has become a widely used fabrication method for scaffolds, facilitating the accurate mapping of the 3D structure of bone defects [1]. In this research,...
Corneal disorders, including trauma, infections, and degenerative diseases, are among the major causes of blindness. Due to the global shortage of donor corneas and risks associated with transplantation, there is a growing interest in developing bioprinted corneal implants as an alternative therapeutic approach.
The aim of this study was to evaluate an alginate-based bioink. In the first...
Introduction
Osteosarcoma (OS) is a rare and aggressive primary bone tumor affecting children and adolescents. Traditional 2D models fail to replicate the complexity of boneโtumor interactions.
Aim: to develop a hybrid 3D osteosarcoma model replicating interactions between healthy and malignant bone tissues.
Methods
Healthy bone-mimicking scaffolds were fabricated using TCP-enriched (20...
Introduction:
Replicating physiologically relevant tissue environments in vitro requires both sophisticated biological design and reliable scalable fabrication. Current approaches often rely on complex biofabrication methods such as stereolithography (SLA) and melt electrowriting (MEW), which are time-intensive, laborious, and lack reproducibility at scale [1,2]. To overcome these...
Introduction
Robotic additive manufacturing (RAM) is currently being explored to overcome the limitations of layer-by-layer additive manufacturing technologies in the fabrication of complex constructs for regenerative medicine (RM). A few successful attempts at using RAM for in-situ extrusion-based bioprinting have been reported, where the fabrication is non-planar but still...
