Conveners
Poster Session II: Poster Exhibition
- Carmelo De Maria (University of Pisa)
- Marco Costantini (Institute of Physical Chemistry Polish Academy of Sciences)
Poster Session II: Poster Exhibition
- Marco Costantini (Institute of Physical Chemistry Polish Academy of Sciences)
- Carmelo De Maria (University of Pisa)
Description
posters are on display whole day on Tuesday and Wednesday
In this study, we explore a hybrid biofabrication approach combining embedded 3D bioprinting and electrospinning to create bifurcated vasculature. A bifurcated graft is essential during open surgical repair when the aorto-iliac part of the vasculature is diseased. However, fabricating a tissue-engineered vascular graft (TEVG) that closely mimics the native blood vessel network presents...
Introduction and Aim
The in vitro generation of functional vasculature remains a major challenge. Current models use adult cells to mimic the three layers of native blood vessels. Yet, the coordinated morphogenetic events during vasculogenesis are key for vessel functionality and stability. iPSC-derived mesodermal progenitor cells (hiMPCs) remain viable and undergo vasculogenesis after...
Bone defects arising from trauma, infection, or tumor resection presented a major clinical challenge due to the limited self-repair capacity of large bone defects. In this study, a 3D-printed polycaprolactone (PCL) scaffold incorporating copper-doped natural hydroxyapatite quantum dots (Cu-HA QDs) was designed to accelerate both osteogenesis and angiogenesis. The Cu-HA QDs were produced using...
Introduction
Prosthesis and implants are integral parts of modern healthcare, with silicones widely used for their chemical inertness, tissue-like mechanical properties, and adaptability. However, conventional techniques in silicone processing face limitations in structural complexity and patient specificity. 3D printing has emerged as a promising technique for creating personalized medical...
Introduction
One of the main issues limiting the wide-spread application artificial vascular grafts is a high risk of thrombosis due to elasticity mismatch and kinking hazard1. One of possible solutions to these problems is incorporating 3D printed reinforcement to the design of implant2.
The aim of this study is to inspect the possibility of 3D printing with elastic medical grade materials...
The trachea's complex anatomy presents a challenge in the reconstruction of long-segment tracheal airway defects. Its hierarchical architecture, composed of a fibrous outer layer, several cartilaginous rings providing structural integrity, and an inner mucosal lining, makes it challenging to engineer functional tracheal replacements [1]. The need for viable solutions is ever growing [2] due to...
Introduction
One of the major challenges in tissue engineering of three-dimensional (3D) functional tissues is achieving vascularization, which is critical for developing large, viable, and physiologically relevant in vitro models. Constructs exceeding 40โฏยตm in thickness require vascular networks to sustain cell survival1, and blood vessels play a key role in supporting tissue homeostasis...
Background
Critical-size bone defects (CSDs), resulting from trauma, infection, or tumor resection, pose significant clinical challenges. Autologous bone grafting has limitations like donor site morbidity, necessitating novel strategies. Approaches mimicking endochondral ossification, crucial for bone development and healing, show promise for bone regeneration. Many studies involve seeding...
The efficacy of neural interfaces relies heavily on the interaction between conductive hydrogels and underlying substrates. However, the impact of substrate selection on hydrogel performance and cell viability under electrical stimulation remains under-explored. This study investigates the electrochemical behaviour of gelatin methacryloyl (GelMA)-based hydrogels interfaced with indium tin...
Introduction:
Three-dimensionally (3D)-printed bioceramic scaffolds composed of beta-tricalcium phosphate (ฮฒ-TCP) have demonstrated the ability to support robust bone regeneration in critically sized calvarial defects. This bone formation is facilitated through two key biological mechanisms: osteoconduction, which guides new bone growth along the scaffold, and potentially dura-mediated...
Introduction
Depression affects over 350 million individuals globally, with 20โ30% developing treatment-resistant depression (TRD), a major contributor to suicide risk. Existing preclinical models inadequately recapitulate the complexity of the human neurovascular unit (NVU) and bloodโbrain barrier (BBB), thereby limiting the advancement of effective therapeutics. The objective of this study...
Introduction
Biopolymers are widely used in biomedical applications due to their superior biocompatibility and customizable degradability compared to conventional biometals. Among them, polycaprolactone (PCL) is a biocompatible thermoplastic polymer with mechanical properties that make it suitable for a variety of biomedical uses [1]. One of the primary applications of PCL is in bone...
Introduction
The auditory system is essential for speech development, spatial orientation, and communication; dysfunction leads to hearing loss, affecting over 466 million individuals globally, making it one of the top five leading causes of the most years lived with disability [1]. Conductive hearing loss frequently arises from tympanic membrane perforation or ossicular chain disruption,...
Introduction:
Globally, almost two million bone transplants are performed using traditional methods like metallic implants and bone grafts that have their limitations. In this scenario, BTE has emerged as an advanced field to replace the conventional method by allowing a living tissue to be created within biological framework. Over the past few years, GelMA based hydrogels have been widely...
Introduction
Cell carriers are being utilized in cell culture applications that demand efficiency and large volumes of cells, by utilizing the carriers high surface area in a relatively low volume [1]. Hydrogel microgels, which have been extensively utilized in tissue engineering could offer a platform to build tissue [2], but usually scalability of hydrogel microparticle production and a...
Introduction
Functional regeneration of musculoskeletal tissues requires engineered grafts that mimic the heterogenous and anisotropic structure and mechanics of the native tissue. Existing strategies fail to produce tissues that mimic this structural complexity, often leading to deficits in mechanical properties and repair failure in vivo. 3D bioprinting allows for the freeform patterning of...
Introduction
Bone is a dynamic tissue that experiences a wide range of forces during regular daily locomotion. This environment of dynamic strain strongly influences the architecture of the extracellular matrix, and it can impact the rate that bone adapts or recovers after an injury 1,2. Cell research is commonly performed in mechanically static conditions in the base of well plates, yet this...
Introduction
Cardiovascular diseases (CVDs) remain leading cause of mortality worldwide, creating a pressing demand for engineering vascular grafts that can restore or replace damaged blood vessels1. Traditional fabrication techniques often lack the resolution, speed, and geometric complexity required to accurately replicate the intrinsic architecture of native vascular tissues2. In this...
Aphanothece sacrum polysaccharides (ASP) are sulfated polysaccharides derived from a cyanobacterium known as Suizenjinori, which is native to Japan. ASP has potential in food, cosmetics and medical treatments due to its high water retention, anti-inflammatory properties and hydrogel formation ability. Conventional ASP hydrogels are obtained by ionic crosslinking with metal or by physical...
Introduction: Large bone defects present major clinical challenges due to insufficient regenerative capacity. Conventional approaches such as autografts are limited by donor site morbidity and immune complications. Bone tissue engineering (BTE) offers a promising alternative by providing biomimetic scaffolds capable of supporting cellular growth, vascularization, and osteogenesis. Recent...
The neuromuscular junction (NMJ) mediates the transfer of neural signals to skeletal muscle fibers, enabling muscle contraction. The structural organization of the NMJ is critical for efficient signal conduction, and disruptions in its morphology are associated with neuromuscular disorders. However, conventional in vitro models, including 2D culture systems and animal models, offer limited...
Tendon tissue engineering remains a critical challenge due to the need for biomaterials that simultaneously support mechanical load bearing and guide lineage specific cellular differentiation. To address this, we designed a hybrid scaffold system that spatially integrates mechanical reinforcement and tenogenic bioactivity, aiming to mimic native tendon properties more closely than conventional...
Introduction
Cardiovascular diseases (CVDs) are the leading cause of death globally, accounting for approximately 1.6 million deaths annually [1]. Among them, myocardial infarction (MI) is particularly concerning due to its high incidence, mortality, and adverse prognosis. These challenges are amplified in ageing populations, making MI a growing public health issue. Current treatment...
Introduction: Globally, ovarian cancer (OC) ranks as the most common cancer among gynecological malignancies. Recent research highlights the critical importance of the perioperative phase in deterring tumor recurrence. During this time, chemotherapy is the predominant treatment method, and numerous drugs have been developed for this purpose [1,2]. However, a major challenge is sustaining the...
3D bioprinting is a key methodology in biofabrication, enabling precise spatial placement of cellular, polymeric, organic, and inorganic components to construct three-dimensional biological structures. Extrusion-based bioprinting is widely used, as it supports the printing of hydrogels across a broad viscosity range, accommodates the inclusion of cells and cell spheroids for large-scale...
Introduction/Objectives
Injectable hydrogels can support the body's innate healing capability following defect or lesion removal by providing a temporary matrix for host cell ingrowth and regeneration. However, the clinical adoption of current injectable systems remains low due to issues like product dislodgment during administration, nanoporous structures that limit cell infiltration, and...
Abstract:
Introduction:
Islet transplantation is a promising therapeutic avenue for Type 1 Diabetes Mellitus (T1DM),
yet its efficacy is limited by inadequate extracellular matrix (ECM) support and
microenvironmental cues that affect beta-cell viability and insulin secretion. Furthermore,
continuous and accurate monitoring of insulin release is essential for evaluating...
Using natural polymers as biomedical materials for diverse clinical applications has consistently been a focus for scientists. Natural polymers have properties tailored to the specific needs of living organisms and, as a result, have interesting properties of the relevant tissues and are similar to the extracellular matrix. Among the various natural polymers, bacterial cellulose has been...
Idiopathic pulmonary fibrosis (IPF) is an age-associated disorder characterized by progressive fibrosis of lung tissue, with TGF-ฮฒ1 acting as a critical pro-fibrotic factor. Recent studies highlight that epithelial-fibroblast crosstalk, particularly involving senescent lung epithelial (LE) cells, plays a pivotal role in fibrosis progression. Moreover, the extracellular matrix (ECM) and tissue...
Introduction: The purpose of this study was to develop an in vitro 3D model of ovarian cancer, which could help investigate potential anticancer drugs and factors related to angiogenesis. Experimental models that capture tumor structure and incorporate angiogenic processes are in high demand. Angiogenesis is a key stage in tumor development, playing a vital role by supporting blood vessel...
The role of collagen in regenerative medicine therapies is gaining significant attention. Collagen, particularly in its native, fibrillar form, is essential for creating 3D models that accurately mimic the extracellular matrix (ECM). 3D models in regenerative medicine offer realistic tissue replication, improved cell interactions, personalized treatments, better drug testing, complex tissue...
The skin, being the body's largest and most exposed organ, is susceptible to various injuries. Overcoming challenges in wound healing necessitates innovative solutions. This study focuses on advancing skin tissue engineering by developing a bio-ink based on an extracellular matrix (ECM) derived from Porcine duodenum, rich in nutrients and growth factors (IGF, EGF, TGF ฮฒ-1). Porcine duodenum,...
Introduction
The development of functional 3D tissue models has showed significant improvement over traditional 2D cell cultures, as they better mimic the real cell micro-environment. However, in this context, one of the main bottlenecks relates to the difficulty in integrating fully embedded, complex and perfusable vasculature within 3D scaffolds. Even though numerous strategies have been...
Native tissue formation relies on spatio-temporal patterns of cellular proliferation and differentiation occurring over the course of months. In contrast, most biofabrication processes aim to reconstruct full-scale tissue from scratch. This developmental shortcut leads to the well-documented shortcomings in vascularization or innervation. In this work, we present an alternative approach that...
HYCON: A Hydrogel-based Conformable Electrode Array for Noninvasive Electrophysiological Recording of Brain Organoids
Introduction:
Brain organoids have become an essential tool for modeling human brain development, neurological disorders, and therapeutic interventions. However, designing reliable interfaces for these 3D, delicate structures remains a key challenge. Current...
Tissue adhesives with bioactivity, mechanical integrity, biocompatibility, and rapid sealing are critical for wound healing, especially in severe skin injuries. Traditional suturing/stapling have downsides, such as healing delays, infection risk, and poor aesthetics. Biofabricated tissue adhesives offer minimally invasive, tailored biologically active wound closure. Thus, in-situ bioprinting...
Introduction
Three-dimensional (3D) cell culture techniques have become particularly important for the study of lymphoma cells, which are non-adherent and difficult to culture in traditional two-dimensional (2D) systems, and provide cellular environments that better mimic the in vivo tumour microenvironment. Conventional 2D cultures fail to replicate critical features such as cell-cell and...
Introduction
Melt-electrowriting (MEW) is an additive manufacturing technology with the potential to produce regenerative scaffolds that replicate key aspects of the hierarchical structure of musculoskeletal tissues. As small scale polymeric fibres can be accurately deposited using MEW, highly porous 3D scaffolds with well-defined and repeatable pore dimensions can be produced. Herein, we...
Introduction
Melt electrowriting (MEW) is an additive manufacturing technique in which continuous microfibers are formed from polymer melts using an electric field. The fibers are drawn to a desired thickness by electrostatic forces and deposited on a collector at a different electric potential using a conventional motion system. Due to its origins in tissue engineering, a vast majority of...
ABSTRACT
In this study, we have developed a modified microfluidic T-junction tubing apparatus to fabricate high-throughput hydrogel-encapsulated personalized breast cancer organoids. We have used mineral oil as a continuous phase and decellularized adipose tissue hydrogel (DAT) encapsulated patient-derived cancer cells as a dispersed phase to generate homogeneous organoids. After assembly,...
Introduction
The global burden of cardiovascular diseases, particularly myocardial infarction (MI), continues to rise, driving the need for advanced therapeutic strategies that can restore damaged heart tissue1. Traditional approaches, such as pharmacological interventions and surgical procedures, do not address the fundamental issue of myocardial regeneration. Moreover, the scarcity of donor...
Introduction
A microfluidic device supported by a hydrogel matrix and carbon nanotubes (CNTs) is a promising tool in developing cancer cell research. These innovative lab-on-chip (LOC) systems enable precise microenvironment control, mimicking in vivo conditions to enhance cancer colony growth and, next, cancer treatment. The integration of hydrogels with microfluidic platforms facilitates...
Tissue engineering methods and regenerative medicine innovations rely on scaffold materials and their fabrication methods [1]. Scaffolds should be biocompatible, biodegradable, and possess mechanical properties suitable for tissue engineering while mimicking the natural tissue structure [2]. Additionally new organic or inorganic incorporated materials could improve scaffold hydrophilicity,...
Introduction
Biodegradable and piezoelectric poly(L-lactide) (PLLA) is gaining attention for biomedical applications, especially in the field of tissue regeneration, based on piezoelectrically-induced electrical cell stimulation. (1,2) However, PLLA possess weaker piezoelectric properties than non-biodegradable poly(vinylidene fluoride) PVDF. (3) Enhancing PLLAโs piezoelectricity can be...
Introduction
Osteoporosis (OP) is one of the most common metabolic bone diseases, affecting over 200 million people worldwide. At the cellular level, OP occurs due to an imbalance between resorptive osteoclasts and bone-forming osteoblasts. There are several subtypes of OP, this research focuses on the treatment of post-menopausal osteoporosis.
Methods
The planning of an appropriate in...
Introduction
Corneal injuries are a leading cause of vision loss worldwide due to the corneaโs exposed position and precise optical structure.[1] While clinical transplantation is effective, it is limited by donor availability and graft rejection. Mesenchymal stem cell (MSC) therapy offers a promising alternative, promoting corneal wound healing through cell replacement, immunomodulation, and...
Introduction
Cardiovascular disease (CVD) is the most mortality disease globally, and the effective method of its treatment is still unknown. For this reason, there is growing interest in the use of human induced pluripotent stem cells (iPSCs), which can differentiate into all cells in the human body, including induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) which have the...
Introduction
Additive technologies have propelled the popularity of personalized medicine, expanding the adaptation of various biomaterials for additive processing. Sodium alginate (SA) and chitosan (CS) are natural-based, biopolymers that are characterized by simplicity of preparation, ease of modification, and wide availability, and exhibit great potential in additive manufacturing...
Work Title
MAY THE FORCE SHAPE YOU: HARNESSING PHYSICAL BOUNDARIES IN MSC DERIVED MICROTISSUES TO ENGINEER SCALED UP CARTILAGE GRAFTS
Introduction
Articular cartilage (AC) exhibits a unique zonal architecture essential for joint function, characterized by collagen fiber orientation that shifts from parallel at the surface to radial in the deep zone. Tissue-engineered AC grafts using...
Introduction
Three-dimensional (3D) bioprinting offers high precision and flexibility in constructing in-vitro biological environments. Its role in tissue engineering research and applications has grown significantly in recent years1. When combined with microfluidics, bioprinting enables the production of microgels that provide more stable and diverse environments for cells, thereby reducing...
Musculoskeletal interface injuries, especially at the tendon-bone junctions, present a significant clinical challenge due to their limited ability to regenerate and the structural complexity of native tissues. Current treatment methods often do not restore biomechanical functionality, highlighting the need for advanced biomimetic scaffolds. Our goal is to engineer tissue-specific constructs...
INTRODUCTION
A sustainable alternative to traditional meat is cultivated meat, which is the growth of animal muscle tissue in laboratories. This technology aims to create a cell-laden product that replicates the texture, composition, and structure of conventional meat.[1] However, the hydrogels commonly used in tissue engineering techniques, whether as cast scaffolds or bioinks, often lack...
Introduction
Air leaking is a common problem associated with lung surgery. Surgical sealants implemented to leakage area present a promising solution for postoperative pulmonary air leaks. Particularly, sealant effectiveness depends on mechanical compatibility with the visceral pleura and mismatched mechanical properties can cause leaks, detachment from the tissue, or even tissue damage. A...
Nippi Inc., a Japanese manufacturer of collagen and gelatin, has spent over a century dedicated to improving peopleโs quality of life and developing breakthrough products. Guided by our longstanding philosophy of โQuality the First,โ we remain committed to supporting the future of human well-being as experts in protein engineering.
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At this exhibition, we will showcase our highly...
Introduction
Current treatment for temporomandibular joint (TMJ) disorders consisting of pain mitigation or surgical invasion, remain limited. Conventional scaffold fabrication techniques, such as fused deposition modeling, lack microscale resolution necessary to recreate the architecture of TMJ cartilage. In contrast, melt electrowriting (MEW) is a high-resolution additive manufacturing...
Introduction:
Polyphenols are a diverse group of naturally occurring organic compounds with potent antioxidant properties. These compounds play a crucial role in the prevention of various lifestyle-related diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions [1]. Among them, myricetin (MYR) stands out due to its well-documented health-promoting effects such...
Introduction
Wound healing follows a five-stage process when skin tissue is injured, but factors like wound size and patient health may require additional interventions [1]. Tissue-engineered structures, especially electrospun meshes, offer enhanced regeneration by mimicking the skinโs extracellular matrix, promoting hemostasis, absorbing exudate, and minimizing scarring [2]. However,...
Introduction
In situ bioprinting is a field of tissue engineering that employs the additive manufacturing of soft implants directly into the patient. In this biofabrication technique, the implant is printed layer by layer on the site of repair to achieve the desired anatomy. As the body is not a perfectly flat surface, non-planar algorithms for toolpath generation [1] can help to enhance the...
Printing geometrically complex structures requires the preparation of advanced G-code templates to control the printer accurately. Most available slicers are optimized for FDM printing using thermoplastics, which solidify rapidly upon cooling. In contrast, collagen-based bioinks solidify much more slowly and require precise temperature control during printing to prevent degradation while...
Introduction:
The development of biomaterials for tissue engineering applications requires a delicate balance between biocompatibility and mechanical performance. While ensuring a materialโs compatibility with living tissues is essential to avoid adverse immune responses, mechanical strength and the ability to fine-tune it are equally critical to support tissue regeneration and withstand...
Gelatin Methacryloyl (GelMA) attractes considerable research attention as an important structural
component for bioinks.1 The synthesis of GelMA involves the methacrylation of gelatin, wherein methacryl groups are covalently bonded to the amino groups of lysine residues. The degree of methacrylation (DM) is a critical parameter that significantly affects the physicochemical properties of...
Bone tissue engineering aims to develop biomaterials that can effectively repair and regenerate damaged bone tissue. Polycaprolactone and calcium phosphate, which are used in this field, are among the most widely used biocompatible materials. However, these materials lack antimicrobial properties and are vulnerable to bacterial adhesion and biofilm formation, which can lead to implant failure....
Introduction
Pancreatic Ductal Adenocarcinoma (PDAC) is hallmarked by a dense, collagen-rich stroma, driven by the activation of pancreatic stellate cells (PSCs), which remodel the extracellular matrix (ECM) into a mechanically stiff microenvironment that promotes tumor progression and therapeutic resistance.1,2 To accurately mimic this fibrotic transformation in vitro, we engineered 3D...
Polycaprolactone (PCL) is a synthetic, biodegradable aliphatic polyester widely used in tissue engineering and has been FDA-approved for various medical devices. However, its long-term performance is compromised by undesirable issues such as surface biofilm formation and a foreign body response (FBR)[1]. These adverse biological processes not only reduce scaffold functionality but also trigger...
