Wound healing effects of an acellular skin substitute in third degree burns

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ICE Krakow

ICE Krakow

ul. Marii Konopnickiej 17 30-302 Kraków


Godoi, Manuella (Federal University of Santa Catarina- UFSC/ Biocelltis)


"Burned skin wound treatment is one of the most expensive burdens on healthcare systems worldwide. The severity and extension of burn trauma could elevate the rate of hospitalization, and patients with burn wounds are especially vulnerable to infections. Burn injuries are very challenging to manage since it causes loss of the integrity of large portions of the skin, leading to major disability or even death. 1 In order to address limitations of conventional treatments and solve the problem of scarcity of donor grafts or immunological rejection with allografts, skin substitutes from tissue engineering emerged as an alternative.
In addition, these substitutes can prevent the growth of microorganisms and improves wound microenvironment, aids cellular behavior, reduces inflammation status and enhances tissue regeneration. 2,3 The aim of this research is to develop and evaluate the effects of the ARTSKin™️ dressing in the early stages of treatment after burn. Acellular ARTSKin™️ is a hydrogel-like biomaterial, consisting of 98% water and 2% natural polymeric nanofibers structurally similar to human collagen, mimicking the extracellular matrix, and thus providing a suitable microenvironment for human tissue manufacturing. In vitro studies prove the functional effects of the ARTSKin™️ on cells, the non-toxicity of the biomaterial and its use as support for the cell cultivation in epidermal, dermal and/or dermoepidermal substitutes.
In vivo, ARTSkin was used as a skin substitute to overlie thermal burns induced on the dorsal skin of mice using a preheated brass template. Firstly, burned tissue was debrided and then overlaid with acellular ARTSkin. To create a full thickness burn wound, the brass block was applied at 100ºC for 20 seconds, using a constant pressure. Multimodal analgesia was adopted for pain management. The administration regime of analgesia included preemptive or pre-procedural analgesic administration. 4 Histopathological investigation and microbiome of skin tissue of C57BL/6J mice showed almost accelerated wound closure compared with a standard control group. All the information obtained from this study indicated that the ARTSKin can be considered a promising skin substitute.

1. Jescke, M. G. et al. Nat. Rev. Dis. Prim. 6:11, 1-25 (2020).
2. Swingler, S.et al. Polymers. 13 (3):412 (2021).
3. Ahmed, J. et al. Biotechnol Adv. 41:107549 (2020).
4. Blaise, O. et al. JOVE. DOI: 10.3791/61339-v (2020)."

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