Speaker
Description
Introduction:
One of the most common labor disturbances is the reduction of prenatal blood flow which results in reduction of oxygen level in neonatal brain. This in turn can lead in long term to the development of drug-resistant epilepsy. Main therapy in the case of epilepsy is administration of sedative drugs which alleviate degree of seizures but also decline the child’s cognitive development. Hence, it is an urgent problem to develop a novel therapy that would make the neonatal seizures less intensified with possibility of correct development. Stem cell-based therapy has attracted much attention because of not only its regenerative property but also its long therapeutic time window. The study determines how the secretome of human Mesenchymal Stem Cells (hMSCs) may influence Organotypic Hippocampal Cultures (OHC) derived from NOD SCID mice with Temporal Lobe Epilepsy (TLE) induced using pilocarpine.
Methodology:
Experiments were performed by culturing OHC in MSCs – conditioned media, which was prepared by incubating MSCs in OHC-dedicated medium for 48 hours. MSCs secretome influence on pilocarpine-induced hippocampal tissue damage was carried out by the assessment of OHC morphology, followed by colorimetric cell viability and mortality tests and also the measurement of chosen pro-inflammatory factors in the culture medium and/or OHC lysates.
Results:
Our data report indicates that the MSCs cell therapy is effective in preclinical studies in terms of viability and morphology of OHC amelioration, as well as improvement of immune stage of damaged brain tissue.
Conclusions
We have proven that the pilocarpine mice derived OHC are a viable model to investigate the efficacy of stem cell-based therapies. Easy obtainability of MSCs, combined with no ethical problems in cell collection, no tumorigenicity, low immunogenicity and easy preparation seem to be crucial when translating into the clinic.
Project was supported by National Scientific Center in Poland 2018/31/B/NZ3/01879.
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