Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Chapter Conference 2022

Polyphenols and mesoporous bioactive glasses doped with therapeutic ions as biofunctional additives for PCL-based composites

Not scheduled

20m

ICE Krakow

poster PS08 Antimicrobial biomaterials for bone regeneration

Speaker

Chęcińska, Kamila (Department of Glass Technology and Amorphous Coatings, AGH University of Science and Technology )

Description

Introduction: The growing requirements for tissue engineering results in the design of new materials, including polymer composites with various modifying phases. In addition, active substances can modify the properties of materials, and thus the human body's response. For this purpose, polymer-ceramic composites with various mesoporous glass (MBGs) modifying phases were designed. Numerous literature reports state that ordered mesoporous channels, a large specific surface area and a large pore volume make these glasses ideal candidates as carriers of biologically active substances. In this work, polymer matrix was modified with MBGs doped with therapeutic ions (Cu, Mg). Composites were additionally enriched with sage (Salvia Officinalis L.) extract showing antibacterial, anti-inflammatory and anti-cancer properties.

Methodology: The solvent casting method was used to produce composite films based on PCL. The materials were modified with polyphenolic compounds (PPh) extracted from sage (4.5 wt.%). MBG particles (d50 – 1,5 μm, 30 wt.%) were used as the modifying phase. Composites were analyzed in terms of in vitro bioactivity in simulated body fluid (SBF) by assessment of morphological and chemical changes using SEM/EDX, while kinetics of bioactive layer formation and therapeutic ion release was analyzed using ICP-OES technique. Furthermore, ABTS, DPPH, and FRAP methods were used to evaluate antioxidant activity of composites.

Results and conclusions: All composites showed high bioactivity in contact with SBF. The influence of polyphenols on the formation process of calcium phosphate layer on the surface of materials was noticed. Additionally, ICP-OES analysis showed that the bioactive mesoporous glass made it possible to obtain highly bioactive composite materials capable of releasing therapeutic ions. MBGs affected significantly antioxidant properties of the materials as a result of PPh binding.

This work was supported by the National Science Centre, Poland Grant no. 2017/27/B/ST8/00195 (KCK).

94238166507