Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Chapter Conference 2022

BIOPATTERNING OF 3D CELLULAR STRUCTURES VIA CONTACTLESS MAGNETIC MANIPULATION FOR DRUG SCREENING

Not scheduled

20m

ICE Krakow

poster PS07 Advances in cardiac tissue engineering: in vitro platforms and in vivo regeneration

Speaker

Onbas, Rabia (Department of Bioengineering, Izmir Institute of Technology (IZTECH), 35430 )

Description

"Patterning and manipulation techniques have been used to fabricate 3D cell cultures in tissue engineering. The contactless magnetic manipulation approach is a rapid, simple, and cost-effective method that requires paramagnetic agents [1-3] or magnetic materials [4]. Here, to obtain patterned 3D cellular structures a new alginate-based bio-ink formulation was developed to fabricate 3D cellular structures using contactless magnetic manipulation. 3D cardiac model was obtained by patterning rat cardiomyocytes. Cellular and extracellular components and cardiac-specific markers of patterned 3D cellular structures were indicated successfully. Drug response of patterned 3D cellular structures was evaluated by applying doxorubicin. Patterned 3D cardiac cellular structures showed significantly different drug response compared to conventional 2D cell cultures. In conclusion, this technique provides an easy, efficient, and low-cost methodology to fabricate 3D cardiac structures for drug screening.

References

1. Turker E, Demircak N, Arslan Yildiz A., Biomaterials Science 6, 1745-1753 (2018)
2. Onbas R, Arslan Yildiz A., ACS Applied Bio Materials 4, 1794-1802 (2021)
3. Abdel Fattah A.R. et al., Biomater Sci, 6 (3), 683-694 (2018)
4. Timm D.M. et al., Sci Rep, 3, 3000 (2013)
   "

62825457368