Spatio-temporal emergence of multicellular engineered structures as preclinical models

30 Jun 2022, 16:50
10m
Room: S3 B

Room: S3 B

Speaker

Yesil-Celiktas, Ozlem (Ege University)

Description

Multicellular tissues such as spheroids, organoids can be assembled in vitro from clusters of cells in a spatio-temporal manner, mimicking in vivo physiology and tissue microenvironment, which can leverage pre-clinical evaluation of the efficacy and safety of potential new drugs for diseases such as hereditary, neurodevelopmental, infectious and cancer. In one approach, immortal, primary or differentiated pluripotent cells are seeded in three-dimensional (3D) constructs leading to assembled multicellular structures, whereas the other approach involves the self-organization of cells that are subjected to a variety of external stimuli such as physical, chemical, mechanical and electrical. One of the greatest challenges is the regulation of spatio-temporal organization and/or differentiation of cells within a 3D microenvironment. In this study, the impact of microgravity in self-assembly of cells was evaluated in a bioengineered system that provided controlled dynamic flow conditions, supported growth factor diffusion, metabolite exchange in larger sizes and survival. The formation of multicellular structures was not only investigated on cellular level including morphology, proliferation, adhesion but also on functional level in terms of recapitulating the physiology. Reproducible scaling of these engineered spheroids and organoids in consecutive batches will allow high-throughput screening of emerging therapeutics and combinational therapies as preclinical models at an industrial scale, which is envisaged to accelerate in the near future.

31412745855

Presentation materials

There are no materials yet.