The establishment of developed 3D micro-frame co-culture model to study the neurovascular effects of marine fungal derived citreohybridonol

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ICE Krakow

ICE Krakow

ul. Marii Konopnickiej 17 30-302 Kraków


Sokullu, Emel (Koc University )


"Marine-based novel biomolecules are an emerging set of metabolites for the development of next-generation biomaterials, drugs, and pharmaceutical in vitro platforms [1]. Marine-derived drugs have the potential capacity against different sorts of cancers and isolation of marine-derived natural products has been in the center of attention in developing anticancer drugs because of their strong adaptability and high yield [2]. The usage of naturally synthesized polymers such as gelatine methacrylate could be a promising approach to developing a valid anti-cancer drug delivery system [3].

Here, we investigated the neurovascular potential of the citreohybridonol in neuroblastoma treatment via the 3D micro-frame co-culture technique. 3D microenvironment was constructed by stainless photomasks, frames, and GelMA. Citreohybridonol was isolated from a sponge-derived fungus Penicillium atrovenetum The model was characterized by FTIR and SEM analyses. HUVEC and SH-SY5Y cells were encapsulated in the GelMA within and without citreohybridonol The effects of citreohybridonol on the proliferation capacity of cells were assessed via cell viability and immunostaining assays.

GelMa and 3D culture characterization indicated that cells have been successfully encapsulated as axenic and mixed within/out citreohybridonol. The MTT assay confirmed that the 3D microenvironment was non-toxic for cultural experiments and showed the inhibitory effects of citreohybridonol on SH-SY5Y and induced the proliferation of HUVECs. Finally, immunohistochemical staining demonstrated that citreohybridonol suppressed SH-SY5Y cells and induced the vascularization of HUVECs in mixed 3D cell culture.

Following the results from the current study engineered 3D tissue platforms ensure accurate information for drug screening and diagnostic platforms. Besides, our study indicated that the cost and time of experiments could be decreased, and co-cultivation of different cells could be easily applied. Moreover, the results of this study indicated that citreohybridonol was a unique novel alternative for the treatment of neuroblastoma insists on surgical operations. This study was one of the primary research that used a 3D micro-frame co-culture tissue model and natural product chemistry in drug discovery."


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