Tissue Engineering and Regenerative Medicine International Society (TERMIS) European Chapter Conference 2022

Session

S02 3D in vitro tissue-engineered cancer/disease models – Session II

S02

Jun 28, 2022, 1:30 PM

ICE Krakow

Conveners

S02 3D in vitro tissue-engineered cancer/disease models – Session II

• Rui L. Reis
• Serena Danti

955. Mimicking the tumor stroma-induced vasculature collapse in 3D pancreatic tumor model

Prakash, Jai (University of Twente)

6/28/22, 1:30 PM

invited lecture

"Introduction: Pancreatic ductal adenocarcinoma (PDAC) is one of the most fibrotic tumors, which can possess up to 90% tumor stroma of the total tumor mass (1). The tumor stroma is comprised of cancer-associated fibroblasts (CAFs), extracellular matrix (ECM) and many immune cells. The physical and biochemical characteristics of the tumor stroma control cancer cell proliferation, invasion, and...

2. BOTTOMS-UP BIO-PRINTING OF CELLULARIZED POROUS MICRO-SCAFFOLDS TO ENHANCE CELL PROLIFERATION, VIABILITY AND MIGRATION

Rousselle, Adrien (Inserm UMR 1121 )

6/28/22, 1:50 PM

podium presentation

Extrusion bio-printing is the most direct and inexpensive method for printing three-dimensional cell models. This technique provides interesting solutions to generate more complex architectures than the already existing 3D models but still presents significant drawbacks that once solved will improve its field of application in regenerative medicine and advanced 3D biological models. To print...

8. Post-printing structure formation in bioprinted tissue constructs that mimic the tumor microenvironment

Neagu, Adrian (Victor Babes University of Medicine and Pharmacy Timisoara )

6/28/22, 2:00 PM

podium presentation

Understanding the key players in cancer progression is essential for the development of effective therapies. Aiming to pinpoint the roles of biochemical and biophysical factors involved in malignancy, tissue engineers developed in vitro cancer models of increasing complexity [1]. Three-dimensional (3D) bioprinting techniques were extensively used in this endeavor [2,3] due to their ability to...

10. Bioreactor dynamic organotypic culture of primary liver cancer as a personalised immunocompetent drug screening platform for immuno-oncology

Urbani, Luca (Roger Williams Institute of Hepatology )

6/28/22, 2:10 PM

podium presentation

Introduction: Current primary liver cancer models fail to truly encompass the human tumour immune microenvironment, exacerbating a recognised discord between the preclinical and clinical successes of emerging (immuno)therapeutics. The organotypic 3D culture of human precision-cut tumour slice (PCTS) is a cancer explant model which retains tumour specific histoarchitecture, aetiological...

11. Development of a bioprinted breast cancer model using decellularized mammary glands

Blanco-Fernandez, Barbara (Institute for Bioengineering of Catalonia (IBEC) )

6/28/22, 2:20 PM

podium presentation

Introduction
3D bioprinting has emerged as a promising technology for fabricating artificial tumors as it allows the fabrication of complex models recreating tumor physiology. The importance of the extracellular matrix (ECM) in tumor progression, cancer cells and stromal cells crosstalk and drug resistances, has motivated the development of more biomimetic tumor-ECM bioinks that recapitulate...

12. INVESTIGATION OF BREAST CANCER EPITHELIAL-MESENCHYMAL TRANSITION USING 3D COLLAGEN-BASED MODELS

Sainsbury, Elizabeth (Royal College of Surgeons Ireland )

6/28/22, 2:30 PM

podium presentation

Treatment options for triple-negative breast cancer (TNBC) are limited. Current 2D cancer models fail to accurately model the tumour microenvironment of breast cancer. Alterations to extracellular matrix (ECM) composition have been shown to play a key role in the epithelial-mesenchymal transition (EMT) process involved in breast cancer progression (1). This highlights the need for the...

46. ENGINEERING BIOMIMETIC HUMAN LUNG TUMOR MODELS

Ozturk, Ece (Koc University )

6/28/22, 2:40 PM

podium presentation

Introduction
Lung cancer is the leading cause of cancer mortality with poor prognosis due to late stage diagnosis, drug resistance and high risk of relapse. There is a high need for tissue engineered 3D models that can recapitulate tumor heterogeneity and complexity to understand the cellular mechanisms leading to lung tumorigenesis, metastasis and drug responses. Future of precision oncology...

51. THE BIOMECHANICAL SIGNATURES OF 3D IN VITRO TUMOUR MODELS

Micalet, Auxtine (University College London )

6/28/22, 2:50 PM

podium presentation

INTRODUCTION: Most epithelial cancer cell populations undergo an epithelial to mesenchymal transition (EMT), acquiring a more aggressive phenotype1. Mesenchymal cells are more motile and have the ability to remodel the extracellular matrix. This mechanical interaction with the surrounding matrix can be measured by bulk and single cell force generation. In this study we aim to use 3D in vitro...