Introduction: Advanced strategies in tendon Tissue Engineering might overcome the unsatisfactory results of conventional treatments for tendinopathies [1]. It aims at designing 3D tendon biomimetic scaffolds with adequate physical, mechanical, and biological properties of the native tissue. In this context, 3D tendon biomimetic poly(lactide-co-glycolic) acid (PLGA) scaffolds with highly...
Introduction: Fibroblast populated collagen matrices (FPCMs) are well established as a model for assessing the behaviour of fibroblasts. When used on a fixed substrate, FPCMs have also proven to be a reliable model for the study of tissue healing. Until now, such techniques have only been used with dermal fibroblasts to examine skin healing. Our work will develop a fixed (tethered) FPCM seeded...
In addition to their chemical composition various physical properties of synthetic bone substitute materials have been shown to influence their regenerative potential and to influence the expression of cytokines produced by monocytes, the key cell-type responsible for tissue reaction to biomaterials in vivo. In the present study both the regenerative potential and the inflammatory response to...
Introduction:
One of the main methods to investigate cellular function, including stem cell differentiation, pathological state, and drug responsiveness, is by analysing deposition of extracellular matrix (ECM). Current methods such as Western blot, mass spectroscopy, or immunostaining, either require the removal of cells and processing of the ECM prior to analysis, or only allow for the...
Introduction. Osteosarcoma is the most common type of bone cancer diagnosed especially in children and young adults1. A combination of chemotherapy, radiotherapy and surgery is commonly used to treat this type of cancer2,3. In detail, chemotherapy is based on the use of molecules targeting the high cancer cell proliferation metabolism such as Platinum-based drugs that binds nuclear DNA upon...
INTRODUCTION: Osteoblasts (OBs) and osteoclasts (OCs) interaction have an important role in bone remodeling [1]. Thus, coculture with both bone cells (OB-OC) is needed to achieve a better understanding of the material-cell interaction and the impact on Mg degradation for its potential use as a biomedical material. The aim of this study was to analyze the surface morphology and dynamic changes...
Type 1 diabetes (T1D) is an autoimmune disease that leads to the destruction of pancreatic islets, resulting in the deficiency of insulin secretion. The current treatment is a daily insulin injection regimen, which negatively affects the quality of life for T1D patients and is difficult to manage over an entire lifespan. Therefore, an alternative treatment – islet transplantation within...
Introduction: Biomechanical features and osteogenic properties of magnesium implants make them suitable for orthopedic applications. [1] Commonly used in vivo methods to investigate tissue response, such as histology and gene expression of peri-implant tissue, are terminal procedures that give a limited temporal and spacial view on the organism response to the implant. [2] Thus, systemic...
Introduction:
The in vitro differentiation of bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs) to chondrocytes is mainly driven by the exogeneous administration of transforming growth factor β (TGF-β)[1]. Multiaxial load-induced activation of TGF-β1 (TGF-β1), which is secreted by the cells in a latent form has been shown to drive chondrogenesis[2]. In the present work, we apply a...
Introduction: Electroactive smart materials are increasingly being used for tissue regenerative applications. Piezoelectric polymers, such as poly(vinylidene fluoride) (PVDF), are one type of electroactive materials that generate electrical potential fluctuations simply by applying mechanical pressure to the material, without the need for additional power sources. The use of piezoelectric PVDF...
Introduction. Glioblastoma is a very aggressive type of cancer with a very poor life expectancy for patients and breast cancer often metastasizes into the liver, lungs, brain, and, in 70% of cases, to bones[1,2]. Chemotherapy is largely used to treat cancer and it is based on the use of molecules targeting the high cancer cell proliferation metabolism[3]. Platinum (Pt) and three of its...
Human bone marrow mesenchymal stromal cells (hBMSC) combined with biomaterials are currently used clinically for bone repair, but their bone formation kinetics are heterogeneous. The development of optimized pro-regenerative therapies requires a rigorous understanding of the molecular progression of regeneration. Here we report the divergent molecular events that drive hBMSCs toward fibrosis...
The quality of life of millions of patients has been improved by the development materials for regenerative medicine such as total knee joint prostheses, cardiovascular stents, orthopaedic implants, as well scaffolds for tissues regenerative. The global regenerative medicine market size is expected to reach USD 57.08 billion by 2027, growing at a CAGR of 11.27% over the forecast period.
In...
Introduction: Living tissue is a very complex biological construct made of cells, signaling molecules and multiple extracellular matrix (ECM) components. Each of these elements is equally important to keep the homeostatic balance of tissue and to control the functions of organs. This heterogeneity of living tissues makes their engineering in laboratory conditions challenging 1. Biocompatible...
