Introduction
This work presents a computational model to study release profile of proteins grafted on scaffolds surfaces. As a case study we investigated the behaviour of ICOS-Fc, a bioactive protein able to rebalance the osteoblasts and osteoclasts activities [1], bound to a polyester blend. Then, a three-dimensional (3D) model was implemented to evaluate the release and diffusion of...
Introduction: Pelvic organ prolapse (POP) is a condition in which defects of supportive tissues due to changes in collagen metabolism result in bladder, bowel, and uterus descending into the vagina, which results in a lifetime surgery risk of 10% worldwide [1]. Surgery comprises the plication of healthy connective tissue to cover the defect, which is called native tissue repair (NTR). However,...
Introduction
In addition to the osseointegration of the implant’s surface, a sufficient adhesion of the abutment's surface to the soft tissue is a crucial factor for the longevity of the implant. The soft tissue seal surrounding natural teeth acts as a barrier against deleterious stimuli in the oral cavity, thus preventing bacteria penetration and protecting the underlying alveolar bone....
Abstract
Tissue engineering (TE) combines both cells’ biology and engineering to develop bioactive biomaterials to treat tissue regeneration [1]. Among them, biomaterials with electric conductive properties have shown promising potential as bioactive cell substrates for tissue regeneration, especially for electrically active tissues, such as the skeletal muscle system [2].
Alginate hydrogels...
Nanoparticles have attracted a great deal of interest as non-viral vectors for gene delivery. However, achieving targeted delivery is difficult. Local particle delivery is achieved by exploiting the sensitivity and responsivity of cells to their mechanical environment. Particle surfaces offer the possibility to modify the properties of existing structures and determine cell behaviour through...
Introduction: Immobilization of cell adhesive proteins on the scaffold surface has become a widely reported method for improving the interaction between scaffold and cells [1]. Immobilization is performed using various methods based on chemical binding or physical interaction between biomolecules and polymer. Benefits, as well as weak points of both ways, are under discussion in the literature...
Introduction
Poly(vinyl alcohol) (PVA) may be used in 3D printing as a sacrificial material in the formation of tissue engineering constructs with complicated architectures. Our research aims to combine 3D printing scaffold fabrication based on PVA as a sacrificial material with enzymatic mineralization and chemical crosslinking of hydrogels, which are done by incorporating specific chemicals...
Cartilage is a non-vascular connective tissue composed of specialized cells – chondrocytes. The lack of blood vessels results in limited cartilage self-regeneration capabilities. While various options are available to treat osteoarthritis, no current method can restore the mechanical function of the original hyaline cartilage. Therefore, herein we address, hyaline cartilage regeneration issue...
Here, we present an analysis of nanoscale deformation of PDMS molds in response to heat and pressure during the repetitive molding process of thermoplastic polymers. The width and height of the nano-sized ridges of PDMS molds decreased as the number of replications of thermoplastic polymers increased. Using the precisely controlled deformation of nanostructures in PDMS molds, we demonstrated...
Introduction
Implant related infections result from persistent bacteria adhering to the biomaterial surface before, during, or after surgery, enabling colonization and biofilm formation on the implant [1]. On average, 4% to 10% of implant surfaces are estimated to be contaminated with bacteria, however, the infection rate can be as high as 30% in intensive care units in developed countries,...
Purpose/Objectives: Bioactuator is a structure that responds to external stimuli by combining an artificial flexible structure and a living biosystem (cells and tissues). Here, we generated and experimented with a structure whose movement can be controlled by external signals by attaching myoblasts to an elastic material in the form of a micro-wire for a bioactuator.
Methodology: Micro-wire...
Introduction
Osteoporosis is a disease that interferes with bone homeostasis making the bone weak and susceptible to fractures. One of the areas affected is the pelvis, whose fractures force the patients to undergo invasive surgeries and long bed recovery.
Here, we aim at fabricating an electrospun composite scaffold based on PEOT/PBT and loaded with particles to promote bone regeneration as...
Introduction
Mesenchymal stem cells (MSCs) are widely used in the fields of cell therapy and tissue engineering, due to their wide spectrum of differentiation potential, immunomodulation function and ongoing oxidative stress (OS) reduction. OS impact is often overlooked in these research fields, even though, it is not only responsible for the induction and development of many ailments, e.g.,...
The lack of bioactivity of poly(N-isopropylacrylamide) (PNIPAM) restricts the use in cell-containing systems to the support as thermoresponsive adhesive substratum with no effect on cell differentiation. In this study, layer-by-layer assembly of polyelectrolytes, allows the combination of PNIPAM-grafted-chitosan (PNIPAM-Chi) with bioactive heparin (Hep) to fabricate PNIPAM−modified...
The usage of artificial materials for tissue engineering is well-known. Their use as a vascular prosthesis is being considered more often. One of the most promising materials for use for applications in the cardiovascular system is polyurethane which has a good bio- and hemocompatibility as well as appropriate mechanical properties. However, researchers are still looking for methods to improve...
We have studied synthesis and properties of ultrathin hydrogel coatings with a synthetic extracellular matrix (ECM) analogue: a chimeric peptide comprising a collagen-like peptide block Cys-Gly-(Pro-Lys-Gly)4 (Pro-Hyp-Gly)4 (Asp-Hyp-Gly)4- and the fibronectin RGD block -Arg-Gly-Asp-Ser-Pro-Gly (hereafter CLP-RGD) [1]. For ultrathin hydrogel functionalized with the peptide, we employed...
