Introduction:
Bioreactors are an important tool to study clinical scenarios under more realistic conditions before heading towards preclinical and clinical studies. We previously showed that, using our standard bioreactor (SB), multiaxial load activates transforming growth factor β1 (TGF-β1), and thereby pushes the mesenchymal stromal cells (MSCs) towards chondrogenesis[1]. Such bioreactors...
Introduction
Patient specific, tissue engineered oesophagi represent a possible solution for conditions which currently lack effective therapeutic options, such as long-gap oesophageal atresia1. Histology, the current gold standard for tissue engineering (TE) protocol design, is destructive and 2D, disregarding volumetric information which are essential for confirming the regeneration of the...
Introduction
Optic nerve sheath meningiomas are the second common optic nerve tumor that affect the optic nerve sheath of primarily middle-aged individuals. In general, invasive measures are avoided, as in most situations, surgery yields no good outcome and is often only considered for blind individuals or those with severe proptosis. Regeneration of the optic nerve fibres bearing newly...
Pancreatic islet transplantation is a promising therapeutic advancement in the treatment of type 1 diabetes; however, a major obstacle remains in supporting cell function post-transplant. To identify possible extracellular matrix (ECM) proteins for the functionalization of islet encapsulation devices, we investigated the presence of ECM in native pancreatic tissues and in our EndoC-βH3...
As a valuable resource for cell therapy, spermatogonial stem cells (SSCs) have raised hopes for the treatment of male infertility. Various 3D methods have been developed to produce cellular aggregates and mimic the organization and function of the testis. In order to improve in vitro spermatogenesis, the present study was performed with the aim of forming testicular organoid (TO) from mouse...
Tissue engineering has shown and still shows the potential to improve quality of life, especially in the cell therapy industry. An area of interest is in the in vitro production of red blood cells (RBCs). Blood transfusion is one of the most widespread forms of cell-based therapy and has been in use for over 50 years. However, the World Health Organisation (WHO) aims for each country to have...
Introduction
Cartilage structures can be damaged causing two types of injuries: focal; the lesion is restricted to a concrete zone or diffuse; the lesion affects a broad area of the articular cartilage. This type of lesions appears due to the reduced self-healing capacity of articular cartilage. Diffuse damage is much more difficult to treat. As the damage progresses up to the point where the...
Introduction. The employment of recellularized autologous extracellular matrices (ECM) as scaffold for the reconstruction of complex tissues like skeletal muscle has been recognized as a successful strategy for regenerative medicine. Compared to the application of synthetic patches, which represent the current standard of care for congenital neonatal malformations like omphalocele and...
Polysaccharide-based hydrogels have been developed in the fabrication of multifunctional microenvironments for tissue fabrication. In this study, two opposite charged polysaccharides including chitosan and hyaluronic acid conjugated with phenol moieties (PH) (CH-PH &HA-PH respectively were mixed at different ratios and utilized to enzymatic cross-linking in the presence of H2O2 as an...
INTRODUCTION
The endometrium is a complex, multicellular tissue and is the site of embryo implantation. Dysregulations between embryo-endometrial interactions during implantation result in a variety of pregnancy disorders including recurrent pregnancy loss and implantation failure. Due to a lack of appropriate study models the mechanisms surrounding human embryo implantation are still largely...
Introduction: Tissue engineering approaches appear highly promising for the regeneration of injured bone tissues. This strategy combine three essential components such as: scaffolds, mesenchymal stem cells (MSCs) and growth factors and is based on the culture of stem or progenitor cells on scaffolds in order to generate new bone by osteoinductive cue. Recently bioengineering has been focused...
Adipose tissue plays a crucial role in the human body including heat regulation, energy and hormone homeostasis. The clinical need of adipose tissue is mainly found in reconstructive and plastic surgery, e.g. after tumor resection or to treat deep wounds. The aim of tissue engineering is to build-up vital and functional 3D-tissue models in vitro. Furthermode, adipose tissue is an important...
Introduction: YAP/TAZ complex is considered to be the main player in the mechanotransduction process. It shuttles between cell nuclei and cytoplasm depending on the stiffness of the growth substrate, dimensionality and cell shape. Stem cells in their natural environment, called niche, grow in three dimensional soft tissue but in standard in vitro culture are maintained mostly on flat stiff...
Introduction: Tissue decellularization methods allow obtaining new sources of biological scaffolds obtained from natural tissues and organs1,2. Although decellularized scaffolds can be useful, it is well known that their regenerative potential is significantly improved after recellularization. In the present work, we evaluated the potential of several cell sources to recellularize a xenograft...
Introduction
In-vitro models advanced significantly with the introduction of 3D culture technology providing a physiologically relevant microenvironment. While tissue morphology and functionality have greatly improved, bioengineered tissue models often lack long-term stability with a decrease in cell viability and tissue integrity, preventing their use for chronic exposure and long-term...
Introduction: Bone tissue engineering is a promising therapeutic approach for bone reconstruction, which has been successfully applied in clinical trials. NVDX3 is an allogenic and off-the-shelf candidate advanced therapy intended for use as bone void filling materials. It is a lyophilized, terminally sterilized powder derived from a 3-D cell product containing extracellular matrix and...
Introduction: The cornea which is avascular and transparent tissue is the outer layer of the eye, accepting optical stimuli, and transferring electrochemical signals to the brain. Moreover, the cornea performed a key role in physical barriers against external risks. However, the tissue can be easily damaged and get the disease due to anatomical location. To test irritancy harmful chemicals,...
INTRODUCTION
Bionic implants have been widely used in the clinic to replace or restore impaired neurological function [1]. However, conventional devices rely on stiff metallic electrodes, which often trigger inflammatory responses that hinder longevity and performance [2]. Although different biomaterials have been investigated to dampen the mechanics of stiff electrodes, the establishment of...
