"Introduction
Most of the efforts in creating vascular structures using 3D printing have been concentrated on extrusion type of printing. However, mimicking vascular structures such as arterioles (100 µm diameter) is difficult due to the limitations in resolution that extrusion printing has. Inkjet 3D printing is a droplet-based type of printing that has resolution of 30 µm which can achieve...
"INTRODUCTION
Vascularization remains a critical factor for the success of various applications in the biomedical field. Insufficient blood vessel systems often limit the availability of replacement tissues via TERM.[1] Hence, there is a great interest in materials that potentially improve vascularization. The mechanical properties of hydrogels strongly influence cell behavior and...
"Purpose: Tissue engineering is currently on advanced stage of development which gives a possibilities for novel strategy of personal treatment of type 1 diabetes. AIM: In the following study, a bioink based on ECM derived from decellularization of porcine pancreas was applied for 3D bioprinting.
Methods: The SCID (n=60) and BALB (n=20) mice were used as a model for in vivo study. Porcine...
"Introduction: In the last decade many advancements have been made in engineering tissues that function as replacement for malfunctioning or damaged tissues and organs(1–3). However, current methods are limited to either thin tissues such as skin and bladder(4), or small sized tissues used in small rodent models(2). Scaling these tissues up towards clinical relevant sizes currently remains a...
Introduction
Microfluidic cultivation platforms significantly improve the supply of tissue models in vitro. However, a key challenge is the sufficient distribution of oxygen and nutrients within a 3D construct. Here we demonstrate the use of collagen hollow filaments as a functional unit for the generation of a vascular structure in tissue engineering applications.
Methodology
The hollow...
"Introduction
Tissue engineering employs a variety of different cell friendly platforms capable of supporting and enhancing cell growth and proliferation [1]. Bone and endothelial tissue interaction is closely connected since bone formation depends on vascularization [2]. This work reports on the fabrication of microspheres as biocompatible carriers for the controlled delivery of the growth...
"The search for new or alternative materials for use in bone tissue engineering (BTE) is one of the main challenges. Whey protein isolate (WPI) as a by-product of cheese manufacturing is inexpensive and widely available. Heat treatment of an aqueous solution of WPI above 60 °C results in the formation of a three-dimensional hydrogel network without the use of additional chemical cross-linking...
Functional 3D tissue models are a potentially valuable tool to provide more transferable test results regarding basic research, drug development or other applications than classic 2D cell culture. The biggest bottleneck in the generation of functional 3D tissue models is the incorporation of a reproducible and perfusable vascularization, which on the one side mimics the in vivo hierarchy...
"INTRODUCTION: Regenerating large tissues requires an intimate supply of the host vascular network, a slow process leading to low viability of the regenerating cells. A solution to this obstacle is the generation of prevascularized tissue engineered constructs. Since Endothelial (ECs) and Mural (MCs) cells, such as smooth muscle cells (SMCs), and pericytes (PCs), are the cellular components of...
There is a growing interest in fabrication of bioinks which on one hand biocompatible and on the other hand possess mechanical properties which would allow to fabricate stable constructs capable to survive for a long time after transplantation. Although choosing appropriate material is essential for bioprinting, there is however, another, equally important issue extensively studied nowadays –...
"Introduction: Promoting and stabilizing microvascularization in engineered tissues and organs is still challenging for the available bioengineering technologies. Extracellular matrix (ECM)-based hydrogels have been extensively employed in microvascularization strategies thanks to their soft mechanics and native-like structure. Nonetheless, their bioactivity and physico-mechanical properties...
"Introduction
Anatomically-correct models of human skin are highly desired by both biomedical sciences and industry. Organotypic skin cultures, termed full-thickness skin equivalents, including fibroblasts and keratinocytes, thus forming dermis and epidermis are in use. However, a more detailed model of the skin is difficult to set up due to its cellular heterogeneity, complex vascular...
