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Alzheimer’s disease, a neurodegenerative disorder with unmet therapeutic needs, prompts exploration of electromagnetic interventions [1-2]. We have studied the cell proliferation in mouse brain slices under a long hour RF exposure in an engineered wideband system (0.7-3 GHz) inside an incubator. The brain slices of Wild-type B6129 mice were exposed to pulsed microwaves (6% duty cycle) over a duration of 24 hours at 918 MHz with an averaged SAR of 0.3 W/Kg and at 1800 MHz with a range of averaged SAR values: 0.91W/Kg, 1.38W/Kg, 1.524W/Kg respectively, followed by 5-Ethynyl-2'-deoxyuridine assay to label proliferating cells.
Quantitative analyses revealed a striking frequency-SAR dependency of cortical cell proliferation. Edu assay showed no cell proliferation in control group, whereas RF radiation at 918 MHz (average SAR: 0.3 W/Kg) induced robust proliferation (13 Edu+ cells/mm², p<0.01). At 1800 MHz, the lower averaged SAR (0.91 W/Kg) yielded minimal cell proliferation effect (1 Edu+ cell/mm²), while the higher averaged SAR values of 1.38W/Kg and 1.524 W/Kg progressively increased cell proliferation (5 and 15 Edu+ cells/mm², respectively), suggesting a dose-dependent trend despite lacking statistical significance (p＞0.05).
These results indicate that an averaged SAR of 0.3 W/Kg at 918 MHz is a potent cortical cell proliferative stimulus, while a much higher averaged SAR of 1.524 W/Kg at 1800 MHz is required to achieve a similar cortical cell proliferation level. This study has established pulsed microwaves as a feasible modality for cortical regeneration with frequency and SAR dependency.
