Speaker
Description
The potential biological effects of 5G millimeter-wave (MW) exposure, particularly in combination with solar ultraviolet (UV) radiation, remain largely unexplored. This study investigates the impact of consecutive exposure to 26 GHz MW and solar UV radiation on oxidative stress and reactive oxygen species (ROS) production in HaCaT keratinocytes in vitro. HaCaT cells were maintained under standard conditions and irradiated by 400 J/m2 effective dose of UV radiation using a solar simulator, which was immediately followed by a 2-hours exposure to 26 GHz MW at 5 or 10 W/m2, as consecutive exposure. ROS levels were measured using H2DCFDA fluorescence, while oxidative DNA damage was assessed using the FPG-modified alkaline comet assay. Three independent experiments were conducted for each genotoxic endpoint.
The results indicate that UV radiation alone significantly increased ROS production and oxidative DNA damage, as expected. However, consecutive exposure to 26 GHz MW did not cause any additional effect compared to UV treatment alone. Furthermore, MW exposure alone did not induce oxidative stress or ROS production relative to sham and negative controls. Statistical analysis is ongoing, and final results will be presented at the meeting.
These findings suggest that 26 GHz MW at the tested exposure levels does not enhance or mitigate UV-induced oxidative stress in HaCaT keratinocytes. This study contributes to the growing body of research on potential biological effects of 5G MW exposure and highlights the need for further investigations into long-term effects and real-world exposure scenarios.
