Speaker
Description
We developed an in vitro exposure system for evaluating biological effects of intermediate frequency magnetic fields (IF-MF), focusing on frequencies below 100 kHz.
Our exposure system incorporates a water-cooled magnetic field generator capable of producing stable 85 kHz fields up to 6.1 mT. The system features a specially designed culture vessel that maintains standard cell culture conditions (37±1°C, 5% CO2) while accommodating five 35-mm culture dishes. System monitoring includes thermal imaging and current measurements to ensure stable exposure conditions.
Initial validation using mouse lymphoma cells (L5178Y) demonstrated that the system maintains appropriate culture conditions comparable to standard CO2 incubators, with no significant differences in cell proliferation between exposed and control conditions.
The system is currently being used for genotoxicity assessment using the Mouse Lymphoma Assay (OECD TG490) as a model protocol. Future improvements include implementing a sham exposure device and detailed assessment of induced electric field distribution. With further refinements and data accumulation, this protocol may contribute to evaluation methods for biological effects of IF-MF exposure.
