Speaker
Description
This study investigates the impact of 5G radiofrequency electromagnetic fields (RF-EMFs) on DNA damage in skin cells exposed to ultraviolet (UV) radiation. The human keratinocyte cell line HaCaT and the mouse melanoma cell line B16 were exposed to UVA or UVB radiation, followed by RF-EMF exposure at 3.5 GHz and 28 GHz (4 W/kg SAR for 24 hours). The effects on cell viability, DNA damage markers, and cellular response to DNA repair were assessed. While RF-EMF exposure did not affect cell viability, UV exposure significantly decreased cell viability and increased the levels of γH2AX (a DNA damage marker) and reactive oxygen species (ROS) in both cell lines. Notably, exposure to RF-EMFs after UV exposure reduced γH2AX levels and ROS production. Additionally, UV-induced activation of p38 MAPK was significantly reduced by RF-EMF exposure. The study suggests that 5G RF-EMFs may influence DNA damage and repair processes by affecting the p38 MAPK pathway, potentially altering the cellular response to UV-induced DNA damage.
