BioEM 2025

Session

Oral Session 4

OS4

24 Jun 2025, 13:30

La Nef (Couvent des Jacobins)

Conveners

Oral Session 4: Advancing Biomedical Applications Through Electroporation

• Olga Pakhomova
• Michal Cifra

296. Evaluation of dielectric properties and study of the impact of reversible electroporation through impedance measurement on human pancreatic tumours, healthy tissues and porcine tissues ex vivo.

Théo Le Berre

24/06/2025, 13:30

The dielectric properties of human tissues are important to consider in the context of several biomedical applications, such as electrical stimulation, radio frequency hyperthermia, pulsed electric-field based treatment and for the development of numerical models covering those applications. In this work, we present an experimental study of human pancreases, both healthy and tumour-bearing, in...

297. Biological Autoluminescence as a Real-Time Monitoring Tool for Yeast Electroporation

Michal Cifra

24/06/2025, 13:45

Biological autoluminescence (BAL) presents a novel, real-time, and non-invasive method for monitoring electroporation in yeast. This study highlights its application in optimizing pulsed electric field (PEF) treatments. Using Saccharomyces cerevisiae as a model, BAL dynamics were analyzed during PEF exposure (2–7 kV cm⁻¹), with results validated against traditional methods like impedance...

298. Microsecond Electric Pulses and DC stimulation: A Promising Approach to Targeting Inflammation

Claudia Consales

24/06/2025, 14:00

The results presented in "Microsecond Electric Pulses and DC stimulation: A Promising Approach to Targeting Inflammation" come from the evaluation of the electrical stimulation protocol set in RISEUP project on macrophages cells. Here we demostrate that not only DC, but also mcirosecond electric pulses can modulate the immune respone

299. Multiphysics and multiscale modeling of virtual stem cells under µsPEFs stimulation

Sara Fontana

24/06/2025, 14:15

Recently, increasing interest has been directed toward the use of pulsed electric fields in biomedical applications to promote cell regeneration and differentiation. Central to this is spinal cord injury (SCI) research within the European Project RISEUP, in which an electrified, implantable scaffold-device, able to stimulate stem cells through ultrashort, intense electrical pulses (µsPEFs), is...

300. Pulsed Electric Fields Enhance Cell Killing by Gelonin.

Olga Pakhomova

24/06/2025, 14:30

Gelonin is a ribosome-inactivating protein with high intracellular toxicity but limited cell permeability. Targeted membrane disruption, such as electroporation, enhances its cellular uptake for potential cancer therapy and tissue ablation. We demonstrate a 100- to 1,000-fold increase in gelonin cytotoxicity with pulsed electric fields in T24, U-87, and CT26 cell lines. The effective gelonin...

301. Application of a new coaxial bipolar electrode for the treatment of vertebral metastases: a pilot study in an ovine model

Simona Salati

24/06/2025, 14:45

Spinal metastases represent 90% of spinal masses detected through imaging, necessitating advancements in treatment. Electroporation, a technique using electric energy to alter cancer cell membrane permeability, enhances chemotherapeutic uptake and promotes tumor control. This study aimed to evaluate the safety of using individual and paired electric fields for tissue ablation in healthy bone...